Carica papaya ameliorates allergic asthma via down regulation of IL-4, IL-5, eotaxin, TNF-α, NF-κB, and iNOS levels
Carica papaya ameliorates allergic asthma via down regulation of IL-4, IL-5, eotaxin, TNF-α, NF-κB, and iNOS levels
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DOI：
10.1016/j.phymed.2017.04.009
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发表时间：
2017-08-15
期刊：
影响因子：
7.9
通讯作者：
Javeed, Aqeel
中科院分区：
文献类型：
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作者：
Inam, Asma;Shahzad, Muhammad;Javeed, Aqeel
背景：天然产物作为新药发现的重要来源具有首要的重要性。木瓜叶（Carica papaya leaves，CPL）在传统医学中被用于治疗炎症，目的/假设：本研究采用卵蛋白（ovalbumin-，OVA）诱导的小鼠过敏性哮喘模型，评价CPL提取物的抗炎和免疫调节作用。组-III和-IV分别用CPL提取物和甲基强的松龙（MP）连续治疗7天。在研究结束时，进行肺的组织病理学检查，并在血液以及支气管肺泡灌洗液（BALF）中进行炎性细胞计数。采用逆转录聚合酶链反应（RT-PCR）检测IL-4、IL-5、eotaxin、TNF-α、NF-κ B B和iNOS的mRNA表达水平。我们还发现CPL提取物处理组的血液和BALF样品中的总白细胞计数和分类白细胞计数均受到显著抑制。CPL提取物还降低IL-4、IL-5、嗜酸性粒细胞趋化因子、TNF-α、NF-κ B和iNOS的表达水平。同样，治疗MP，作为参考药物，也显着改善了所有的促炎markers.Conclusion：目前的研究表明，CPL提取物具有抗炎作用，在小鼠过敏性气道炎症模型通过下调IL-4，IL-5，嗜酸性粒细胞趋化因子，TNF-α，NF-κ B，和iNOS的表达水平。
Background: Natural products have a prime importance as an essential source for new drug discovery. Carica papaya leaves (CPL) have been used to treat inflammation in traditional system of medicine.Aim/hypothesis: Current study evaluates the anti-inflammatory and immunomodulatory effects of CPL extract using mouse model of ovalbumin-(OVA) induced allergic asthma.Methods: All the mice were intraperitoneally sensitized and subsequently given intranasal challenge with OVA except the control group. Group-III and -IV were treated for seven consecutive days with CPL extract and methylprednisolone (MP), respectively. At the end of study, histopathological examination of the lungs was performed and inflammatory cell counts were done in blood as well as bronchoalveolar lavage fluid (BALF). The mRNA expression levels of IL-4, IL-5, eotaxin, TNF-alpha, NF-kappa B, and iNOS were measured using reverse transcription polymerase chain reaction (RT-PCR).Results: Results showed significant attenuation of lung infiltration of inflammatory cells, alveolar thickening, and goblet cell hyperplasia after treatment with CPL extract. We also found significant suppression of total and differential leukocyte counts in both blood and BALF samples of CPL extract treated group. CPL extract also alleviated the expression levels of IL-4, IL-5, eotaxin, TNF-alpha, NF-kappa B, and iNOS. Similarly, treatment with MP, used as a reference drug, also significantly ameliorated all the pro-inflammatory markers.Conclusion: Current study shows that CPL extract possesses anti-inflammatory effect in mouse model of allergic airway inflammation by down-regulating IL-4, IL-5, eotaxin, TNF-alpha, NF-kappa B, and iNOS expression levels.