Glycan side chains on naturally presented MHC class II ligands
Glycan side chains on naturally presented MHC class II ligands
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DOI：
10.1002/jms.780
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发表时间：
2005-01-01
影响因子：
2.3
通讯作者：
Stevanovic, S
中科院分区：
文献类型：
--
作者：
Dengjel, J;Rammensee, HG;Stevanovic, S
未知的天然存在的主要组织相容性复合体（MHC）II类糖肽携带复杂的聚糖的分子表征迄今尚未实现，反映了不同的质谱分析中的糖和肽的碎片化特征。人白细胞抗原（HLA）-DR结合肽的分离亲和纯化，通过高效液相色谱法分离和基质辅助激光解吸/电离和电喷雾电离质谱分析。我们能够识别两个自然加工的MHC II类配体，CD 53（122-136）和CD 53（121-136），携带复杂的N-连接的聚糖侧链的组合源和碰撞诱导的碎片的四极飞行时间串联质谱仪。版权所有（C）2005 John Wiley Sons，Ltd。
The molecular characterization of unknown naturally presented major histocompatibility complex (MHC) class II glycopeptides carrying complex glycans has so far not been achieved, reflecting the different fragmentation characteristics of sugars and peptides in mass spectrometric analysis. Human leukocyte antigen (HLA)-DR-bound peptides were isolated by affinity purification, separated via high performance liquid chromatography and analyzed by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. We were able to identify two naturally processed MHC class II ligands, CD53(122-136) and CD53(121-136), carrying complex N-linked glycan side chains by a combination of in-source and collision-induced fragmentation on a quadrupole time-of-flight tandem mass spectrometer. Copyright (C) 2005 John Wiley Sons, Ltd.