Electrogenerated Chemiluminescence Detection of Mercury(II) Ions Based on DNA Probe Labeled with Ruthenium Complex
Electrogenerated Chemiluminescence Detection of Mercury(II) Ions Based on DNA Probe Labeled with Ruthenium Complex
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DOI：
10.2116/analsci.27.193
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发表时间：
2011-02
影响因子：
1.6
通讯作者：
Yaping Li
中科院分区：
文献类型：
--
作者：
Yaping Li
以三（2，2 ′-联吡啶）钌衍生物（钌配合物）为标记物，研制了一种新型的电致化学发光汞离子（Hg^2+）生物传感器。以钌配合物标记的富含胸腺嘧啶的单链DNA（ssDNA）为ECL探针。当另一个富含T的捕获ssDNA自组装到带有巯基的金电极表面，然后在Hg^2+存在下与ECL探针杂交形成双链DNA（dsDNA）结构时，电化学产生了强烈的ECL响应。在1.0 × 10^-6 ~ 1 × 10^-9 M范围内，Hg^2+的ECL强度与浓度呈线性关系，检测限为3.0 × 10^-10 M。在1.0 × 10^−7 M Hg ^2+（n = 5）下，相对标准偏差为4.1%。本工作表明，将富含T的DNA与Hg^2+的强结合与高灵敏度的ECL技术相结合，设计ECL Hg^2+生物传感器是一种很有前途的金属离子测定方法。
A novel mercury(II) ion (Hg^2+) biosensor with electrogenerated chemiluminescence (ECL) detection using tris(2,2′-bipyridyl) ruthenium derivatives (ruthenium complex) as labeling was developed in the prescent work. One thymine (T)-rich single-strand DNA (ssDNA) labeled with a ruthenium complex was taken as an ECL probe. When the other T-rich capture ssDNA was self-assembled onto the surface of a gold electrode with a thiol group, and then hybridized with the ECL probe to form double-strand DNA (dsDNA) structures in the presence of Hg^2+, a strong ECL response was electrochemically generated. The ECL intensity was linearly related to the concentration of Hg^2+ in the range from 1.0 × 10^−6 to 1 × 10^−9 M with a detection limit of 3.0 × 10^−10 M. The relative standard deviation was 4.1% at 1.0 × 10^−7 M Hg^2+ ( n = 5). This work demonstrates that the combination of the strongly binding T-rich DNA to Hg^2+ with the highly sensitive ECL technique to design an ECL Hg^2+ biosensor is a great promising approach for the determination of metal ions.