Metastases-related genes in the classification of liver and peritoneal metastasis in human gastric cancer
Metastases-related genes in the classification of liver and peritoneal metastasis in human gastric cancer
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DOI：
10.1016/j.jss.2005.04.030
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发表时间：
2005-11-01
影响因子：
2.2
通讯作者：
Hirata, K
中科院分区：
文献类型：
--
作者：
Fukui, R;Nishimori, H;Hirata, K
导论.为了鉴定胃癌中的胃癌相关基因，我们对低转移亲本细胞系和已建立的高转移亚系之间的差异基因表达进行了广泛的分析。我们建立了具有高肝转移潜力的新型细胞系AZ-H5 c、NUGC-3 H5和TMK-1H 7，以及具有高腹膜转移潜力的AZ-P7 a、NUGC-3 P4 T和TMK-1 P4 a。这些细胞系分别来源于低转移亲本AZ-521、NUGC-3和TMK-1细胞系。此外，为了研究不同水平的基因表达与胃癌转移潜能的关系，我们使用DNA微阵列研究了每个细胞系中大约2000个表达基因。在高转移的肝和腹膜细胞系中，多种基因表达上调或下调。58个基因，包括转铁蛋白受体，ras相关的rho，和骨桥蛋白，和22个基因，包括载脂蛋白E和apoBin A-submit，在两个或三个肝转移亚系上调和下调。转铁蛋白受体、c-fos、RANTES等19个基因和MAC 25、PISSLRE、RNA聚合酶等26个基因在2 ~ 3个腹膜转移亚系中表达上调或下调。基因表达与胃癌转移的关系至今尚未完全阐明，需要进一步研究以更全面地了解基因在胃癌转移中的作用。我们希望我们建立的胃癌肝转移和腹腔转移的实验模型能为胃癌的进一步研究和基因治疗提供帮助。(c)2005年爱思唯尔公司All rights reserved.
Introduction. With the aim of identifying metastasesrelated genes in gastric cancer, we performed a broad analysis of differential gene expression between low-metastatic parental cell lines and established highly metastatic sublines.Materials and methods. We established novel cell lines, AZ-H5c, NUGC-3H5, and TMK-1H7, with a high potential of liver metastasis, and AZ-P7a, NUGC-3P4T, and TMK-1P4a, with a high potential of peritoneal metastasis. These cell lines were derived from low-metastatic parental AZ-521, NUGC-3, and TMK-1 cell lines, respectively. Furthermore, to investigate different levels of gene expression implicated in metastatic potentials in gastric cancer, we investigated approximately 2000 expressed genes in each cell line using a DNA microarray.Results. Varieties of genes were up-regulated or down-regulated in highly metastatic liver and peritoneal cell lines. Fifty-eight genes, including the transferrin receptor, ras-related rho, and osteopontin, and 22 genes, including apolipoprotein E and inhibin A-submit, were up-regulated and down-regulated in two or three liver metastatic sublines. On the other hand, 19 genes, the transferrin receptor, c-fos, and RANTES, and 26 genes, including MAC25, PISSLRE, and RNA polymerase, were up-regulated and down-regulated in two or three peritoneal metastatic sublines.Conclusion. How gene expression is implicated in gastric cancer metastasis has never been thoroughly explained, and further studies are necessary to understand the involvement of genes in cancer metastasis more thoroughly. We hope that our highly metastatic liver and peritoneal experimental models are helpful for further study and gene therapy of human gastric cancer. (c) 2005 Elsevier Inc. All rights reserved.