Antibiotic susceptibility testing of subgingival plaque samples.
Antibiotic susceptibility testing of subgingival plaque samples.
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龈下菌斑样本的抗生素敏感性测试。
DOI：
10.1111/j.1600-051x.1983.tb01291.x
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发表时间：
1983
影响因子：
6.7
通讯作者：
Socransky,SS
中科院分区：
文献类型：
--
作者：
Walker,CB;Gordon,JM;Socransky,SS
针对从牙周病部位采集的分配的牙菌斑样品测定了几种抗菌剂的体外抑制作用，以帮助选择牙周治疗辅助使用的抗生素。对2组患者进行了采样。其中一组 10 名患有严重晚期疾病的患者接受了牙周治疗，其中包括频繁辅助使用抗生素。第二组由 15 名牙周病较轻的人组成；只有 4 人之前接受过抗生素治疗牙周病。从每位患者身上采集龈下菌斑的细菌样本，并针对一系列抗生素进行测试，以确定哪种药物在抑制细菌生长方面最有效。将每种抗生素掺入 Trypticasesoy 血琼脂中，其浓度相当于推荐口服剂量后在牙龈液或血液中达到的浓度。通过比较含抗生素培养基上回收的细菌数量与基础培养基上回收的细菌总数来确定抑制效果。除氯唑西林外，青霉素是抑制细菌生长最有效的药物。苄青霉素持续抑制在不含抗生素的培养基上回收的 90% 分离株的生长，而氨苄青霉素和阿莫西林经常抑制 99% 或更多的回收细菌。如果患者之前未接受过四环素治疗，那么四环素通常对至少 90% 的分离株具有抑制作用。然而，在先前接受四环素治疗的患者身上采集的样本中，对该药物的耐药性很常见。多西环素是一种四环素衍生物，与四环素相比，抑制的分离株并不多。克林霉素对大多数样品中 90% 或更多的微生物具有抑制作用；和。通常可有效抑制样品中存在大量对四环素具有抗性的分离株。红霉素对从重度患病组样品中回收的分离株相对无效，但对取自较中度患病组的一些样品中的分离株具有抑制作用。在测试浓度下，甲硝唑对两组细菌样本中的分离株基本上无效。没有发现任何单一抗菌剂能够抑制从所有龈下菌斑样本中回收的超过 90% 的细菌，但某些青霉素可能除外。
Thein vitroinhibitory effect of several antimicrobial agents was determined against dispensed dental plaque samples taken from periodontally diseased sites as an aid in the selection of antibiotics for adjunctive use in periodontal therapy. 2 groups of patients were sampled. 1 group of 10 patients with severely advanced disease had received periodontal treatment which included the frequent adjunctive use of an antibiotic. The second group consisted of 15 individuals with less severe periodontal disease; only 4 individuals had been previously treated with antibiotics for their periodontal disease. Bacterial samples of subgingival plaque were taken from each patient and tested against a battery of antibiotics to determine which agent was the most effective in suppressing bacterial growth. Each antibiotic was incorporated into Trypticasesoy blood agar at a concentration equivalent to that achieved in either gingival fluid or blood following recommended oral dosages. The inhibitory effect was determined by comparing the number of bacteria recovered on the antibiotic‐containing medium to the total number of bacteria recovered on the basal medium. Penicillins, with the exception of cloxacillin, were the most effective in inhibiting bacterial growth. Benzylpenicillin consistently inhibited the growth of 90% of the isolates recovered on‐media free of antibiotics while ampicillin and amoxicillin frequently inhibited 99% or more of the bacteria recovered. Tetracycline was generally inhibitory for at least 90% of the isolates if the patients had not been previously treated with this agent. However, resistance to this drug was common in samples taken from patients previously treated with tetracycline. Doxycycline, a tetracycline derivative, did not inhibit significantly more isolates than tetracycline. Clindamycin was inhibitory for 90% or more of the organisms in most of the samples; and. was usually effective in inhibiting isolates in samples which exhibited large numbers of isolates resistant to tetracycline. Erythromycin was relatively ineffective against the isolates recovered from samples from the severely diseased group but was inhibitory to isolates in some samples taken from the more moderately diseased group. Metronidazole, at the concentration tested, was largely ineffective against the isolates in bacterial samples from both groups. No single antimicrobial agent was found to be inhibitory for greater than 90% of the bacteria recovered from all of the subgingival plaque samples with the possible exception of some penicillins.