Topographic specificity of functional connections from hippocampal CA3 to CA1
Topographic specificity of functional connections from hippocampal CA3 to CA1
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DOI：
10.1073/pnas.0308577100
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发表时间：
2004-02-24
影响因子：
11.1
通讯作者：
Callaway, EM
中科院分区：
文献类型：
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作者：
Brivanlou, IH;Dantzker, JLM;Callaway, EM
海马体是一个皮质区域，被认为在学习和记忆中发挥着重要作用。我们对负责这些功能的海马回路的详细组织的大部分了解都来自解剖学研究。然而，这些研究呈现的情况并不完整，因为解剖学通常无法揭示连接的功能特征和重要性。在这里，我们使用生理方法（笼谷氨酸光刺激）来探测小鼠海马切片制备中 CA3 和 CA1 亚区之间功能连接的精细模式。我们记录了 CA1 和 CA3 锥体神经元的细胞内情况，同时以高空间分辨率对整个 CA3 子区域进行光刺激扫描。我们的结果表明，在给定的间隔颞叶水平上，附近的 CA1 神经元接收来自邻近 CA3 神经元的突触输入。因此，CA3 到 CA1 的映射保留了邻居关系。
The hippocampus is a cortical region thought to play an important role in learning and memory. Most of our knowledge about the detailed organization of hippocampal circuitry responsible for these functions is derived from anatomical studies. These studies present an incomplete picture, however, because the functional character and importance of connections are often not revealed by anatomy. Here, we used a physiological method (photostimulation with caged glutamate) to probe the fine pattern of functional connectivity between the CA3 and CA1 subfields in the mouse hippocampal slice preparation. We recorded intracellularly from CA1 and CA3 pyramidal neurons while scanning with photostimulation across the entire CA3 subfield with high spatial resolution. Our results show that, at a given septotemporal level, nearby CA1 neurons receive synaptic inputs from neighboring CA3 neurons. Thus, the CA3 to CA1 mapping preserves neighbor relations.