SUPEROXIDE GENERATION BY DIGITONIN-STIMULATED GUINEA-PIG GRANULOCYTES - BASIS FOR A CONTINUOUS ASSAY FOR MONITORING SUPEROXIDE PRODUCTION AND FOR STUDY OF ACTIVATION OF GENERATING SYSTEM
SUPEROXIDE GENERATION BY DIGITONIN-STIMULATED GUINEA-PIG GRANULOCYTES - BASIS FOR A CONTINUOUS ASSAY FOR MONITORING SUPEROXIDE PRODUCTION AND FOR STUDY OF ACTIVATION OF GENERATING SYSTEM
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DOI：
10.1172/jci109007
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发表时间：
1978-01-01
影响因子：
15.9
通讯作者：
CHOVANIEC, ME
中科院分区：
文献类型：
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作者：
COHEN, HJ;CHOVANIEC, ME
洋地黄素刺激豚鼠粒细胞产生超氧化物(O-2)。连续测定表明，在添加洋地黄素和开始产生O-2之间存在滞后。氧产生系统的激活速率取决于洋地黄素的浓度和温度。氧-2产生的最终线性速率受洋地黄素浓度、温度、pH和外源还原吡啶核苷酸的存在的影响。因此，改变O-2产生系统的激活过程或活性的因素可以影响刺激的粒细胞产生O-2。
Stimulation of guinea pig granolocytes by digitonin results in superoxide (O-2) generation. A continuous assay shows that there is a lag between the addition of digitonin and the onset of O-2 production. The rate of activation of the O-2 generating system is dependent upon the concentration of digitonin and the temperature. The final linear rate of O-2 production is affected by the concentration of digitonin, temperature, pH, and the presence of exogenous reduced pyridine nucleotides. Thus, factors which alter either the activation process or the activity of the O-2 generating system can affect O-2 production by stimulated granolocytes.