Identification, sequence, and expression of caveolin-2 defines a caveolin gene family
Identification, sequence, and expression of caveolin-2 defines a caveolin gene family
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DOI：
10.1073/pnas.93.1.131
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发表时间：
1996-01-09
影响因子：
11.1
通讯作者：
Lisanti, MP
中科院分区：
文献类型：
--
作者：
Scherer, PE;Okamoto, T;Lisanti, MP
Caveolin 是一种 21 至 24 kDa 的整合膜蛋白，是小窝膜的主要成分，Caveolin 直接与异源三聚鸟嘌呤核苷酸结合蛋白（G 蛋白）相互作用，并可以功能性调节其活性。在这里，通过对脂肪细胞来源的富含小窝蛋白的膜进行微测序，鉴定出一种大约 20 kDa 的小窝蛋白相关蛋白 Caveolin-2； Caveolin 被重新命名为 Caveolin-1，Caveolins 1 和 2 在大多数方面都很相似。 Caveolin-1 和 Caveolin-2 的 mRNA 在白色脂肪组织中表达最丰富，并在脂肪细胞分化过程中被诱导。 Caveolin-2与caveolin-1共定位，表明caveolin-2也定位于caveolae。然而，caveolin-1和caveolin-2与异源三聚体G蛋白的功能相互作用不同，这可能解释了为什么caveolin-1和-2在单个细胞内共表达。
Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes, Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity, Here, an approximate to 20-kDa caveolin-related protein, caveolin-2, was identified through microsequencing of adipocyte-derived caveolin-enriched membranes; caveolin was retermed caveolin-1, Caveolins 1 and 2 are similar in most respects. mRNAs for both caveolin-1 and caveolin-2 are most abundantly expressed in white adipose tissue and are induced during adipocyte differentiation. Caveolin-2 colocalizes with caveolin-1, indicating that caveolin-2 also localizes to caveolae, However, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell.