Increased dopamine clearance in the non-lesioned striatum of rhesus monkeys with unilateral 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) striatal lesions.
Increased dopamine clearance in the non-lesioned striatum of rhesus monkeys with unilateral 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) striatal lesions.
复制标题
单侧 1-甲基-4-苯基-1,2,3,6-四氢吡啶 (MPTP) 纹状体损伤的恒河猴非损伤纹状体中多巴胺清除率增加。
DOI：
10.1016/0304-3940(94)11223-6
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发表时间：
1995
影响因子：
2.5
通讯作者：
Gash,DM
中科院分区：
文献类型：
--
作者：
Cass,WA;Gerhardt,GA;Zhang,Z;Ovadia,A;Gash,DM
使用体内电化学来检查正常对照猴尾状核以及单侧 1-甲基-4-苯基-1,2,3,6-四氢吡啶 (MPTP) 治疗猴的未损伤和损伤尾状核中局部应用多巴胺的清除率。在麻醉动物中使用 Nafion 涂层碳纤维电极连续进行计时电流记录，同时从电极附近（250-300 μm）的微量移液器压力喷射校准量的多巴胺。从 MPTP 损伤的尾状核记录的多巴胺信号比从对照和治疗动物的非损伤侧记录的多巴胺信号具有更大的幅度和时间进程，表明损伤尾状核中高亲和力摄取的损失。然而，与对照尾状核相比，从治疗动物的对侧记录的信号中，时程更快，清除率更大。这表明 MPTP 治疗动物的未病变侧的高亲和力多巴胺摄取上调。这种变化可能代表一种补偿机制，试图维持病变侧和非病变侧之间细胞外多巴胺水平的平衡。
In vivo electrochemistry was used to examine the clearance of locally applied dopamine in the caudate nuclei of normal, control monkeys and in the non-lesioned and lesioned caudate nuclei of unilateral 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated monkeys. Chronoamperometric recordings were continuously made using Nafion-coated carbon fiber electrodes in anesthetized animals while a calibrated amount of dopamine was pressure ejected from a micropipette adjacent (250–300 μm) to the electrode. The dopamine signals recorded from the MPTP-lesioned caudate nuclei had a greater amplitude and time course than those recorded from both the controls and from the non-lesioned side of the treated animals, indicating a loss of high-affinity uptake in the lesioned caudate. However, the time course was faster, and teh clearance rate greater, in signals recorded from the contralateral side of the treated animals when compared to control caudate nuclei. This suggests that there is an up-regulation in high-affinity dopamine uptake in the nonlesioned side of the MPTP-treated animals. This change may represent a compensatory mechanism that is attempting to maintain a balance in extracellular dopamine levels between the lesioned and non-lesioned sides.