Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization.
Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization.
复制标题
使用电子轰击电离在等离子体中发现的生理化合物的气相色谱/质谱分析中使用叔丁基二甲基甲硅烷基化。
DOI：
10.1016/0003-2697(84)90431-7
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发表时间：
1984
影响因子：
2.9
通讯作者：
Haymond,MW
中科院分区：
文献类型：
--
作者：
Schwenk,WF;Berg,PJ;Beaufrere,B;Miles,JM;Haymond,MW
报道了用N-甲基-N-（叔丁基二甲基硅基）三氟乙酰胺制备一些有机化合物（选定的氨基酸、α-酮酸、酮体、游离脂肪酸、尿素、甘油、乳酸盐和丙酮酸盐）的叔丁基二甲基硅基衍生物。这些衍生物对于涉及使用稳定同位素和选择离子监测的气相色谱/质谱分析特别有用，因为在低于分子离子的57个质荷单位处总是存在足够丰度的峰，并且是一个叔丁基基团损失的结果。在每一种情况下，这个片段都包含了原始化合物的整个骨架，这使得单独使用这些化合物的电子碰撞电离或在任何不可交换的位置用稳定同位素标记时可以轻松分析。
The use of N-methyl-N-(t-butyldimethylsilyl)trifluoroacetamide to prepare the t-butyldimethylsilyl derivatives of a number of organic compounds (selected amino acids, α-keto acids, ketone bodies, free fatty acids, urea, glycerol, lactate, and pyruvate) is reported. These derivatives are particularly useful for gas chromatographic/mass spectrometric analysis involving the use of stable isotopes and selected ion monitoring, since a peak of sufficient abundance at 57 mass/charge units below the molecular ion was always present, and was the result of the loss of one t-butyl group. In each case, this fragment contained the entire skeleton of the original compound, which permitted easy analysis using electron-impact ionization of these compounds alone or when labeled with stable isotopes in any nonexchangeable position.