Cloned and expressed rat Ca2+-sensing receptor.
Cloned and expressed rat Ca2+-sensing receptor.
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发表时间：
1996
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影响因子：
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通讯作者：
M. Ruat;A. Snowman;L. D. Hester;S. H. Snyder
中科院分区：
文献类型：
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作者：
M. Ruat;A. Snowman;L. D. Hester;S. H. Snyder
我们已经在中国仓鼠卵巢细胞中稳定表达了大鼠脑Ca2+传感受体的cDNA。刺激磷脂酰肌醇水解和花生四烯酸 (AA) 释放对 Ca2+ 表现出显着的协同反应，希尔系数为 4-5。低于 1.5 mM Ca2+ 阈值时未检测到磷脂酰肌醇和 AA 反应。 Mg2+ 表现为部分激动剂，其最大肌醇磷酸盐和 AA 反应只有 Ca2+ 显示的一半，且浓度-反应斜率更浅。在 1.5 mM Ca2+ 存在的情况下，Mg2+ 增强磷酸肌醇和 AA 反应的效力意味着临床条件下达到的血清 Mg2+ 浓度将影响 Ca2+ 感应受体。
We have stably expressed cDNA for the rat brain Ca2+ sensing receptor in Chinese hamster ovary cells. Stimulation of phosphatidylinositol hydrolysis and arachidonic acid (AA) release displayed markedly cooperative responses to Ca2+ with Hill coefficients of 4-5. Both phosphatidylinositol and AA responses were not detected below a threshold of 1.5 mM Ca2+. Mg2+ behaved as a partial agonist with only half the maximal inositol phosphate and AA responses displayed by Ca2+ and with a more shallow concentration-response slope. The potency of Mg2+ in augmenting inositol phosphate and AA responses, in the presence of 1.5 mM Ca2+, implies that serum Mg2+ concentrations attained in clinical conditions will influence the Ca2+-sensing receptor.