A genetic linkage map of azuki bean constructed with molecular and morphological markers using an interspecific population (Vigna angularis x V-nakashimae)
A genetic linkage map of azuki bean constructed with molecular and morphological markers using an interspecific population (Vigna angularis x V-nakashimae)
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DOI：
10.1007/bf00224059
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发表时间：
1996-10-01
影响因子：
5.4
通讯作者：
Kamijima, O
中科院分区：
文献类型：
--
作者：
Kaga, A;Ohnishi, M;Kamijima, O
利用赤小豆与其野生近缘中豆(V.nakashimae)种间杂交F-2群体，利用分子和形态标记构建了小豆遗传连锁图谱。共有132个标记(其中108个为随机扩增多态性DNA标记，19个为RFLP标记，5个为形态标记)定位于14个连锁群，覆盖1250 cM，其中10个为非连锁群。在连锁群2、8和12中发现了表现扭曲分离的标记群。利用20个常见的RFLP标记，将Auki连锁图谱与绿豆和豌豆的连锁图谱进行比较，发现一些标记属于各自图谱的相同连锁群，表明这些连锁块在3个豌豆种之间是保守的。该图谱为分子标记辅助选择和研究豌豆属植物的基因组结构提供了工具。
A genetic linkage map of azuki bean (Vigna angularis) was constructed with molecular and morphological markers using an F-2 population of an interspecific cross between azuki bean and its wild relative, V. nakashimae. In total, 132 markers (108 RAPD, 19 RFLP and five morphological markers) were mapped in 14 linkage groups covering 1250 cM; ten remained unlinked. The clusters of markers showing distorted segregation were found in linkage groups 2,8 and 12. By comparing the azuki linkage map with those of mungbean and cowpea, using 20 RFLP common markers, some sets of the markers were found to belong to the same linkage groups of the respective maps, indicating that these linkage blocks are conserved among the three Vigna species. This map provides a tool for marker-assisted selection and for studies of genome organization in Vigna species.