Vascular endothelial growth factor principally acts as the main angiogenic factor in the early stage of human osteoblastogenesis
Vascular endothelial growth factor principally acts as the main angiogenic factor in the early stage of human osteoblastogenesis
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DOI：
10.1093/jb/mvg081
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发表时间：
2003-05-01
影响因子：
2.7
通讯作者：
Ninomiya, Y
中科院分区：
文献类型：
--
作者：
Furumatsu, T;Shen, ZN;Ninomiya, Y
血管内皮生长因子（VEGF）介导的血管生成是骨形成所必需的。然而，VEGF在成骨细胞发生过程中对成骨细胞的影响仍存在争议。本研究的目的是阐明人骨髓间充质干细胞（MSCs）来源的成骨细胞之间的关系。和VEGF -在成骨细胞分化早期。低浓度地塞米松持续刺激MSCs成骨细胞分化及VEGF 121 mRNA的表达。成骨细胞分泌的VEGF也随着成骨细胞的形成而沿着增加。神经纤毛蛋白-1，主要结合VEGF 165，在成骨细胞形成早期的所有阶段都被检测到，但VEGF受体-1和-2在RT-PCR分析中未被检测到。在本研究中，VEGF对成骨细胞的增殖没有直接影响。然而，成骨细胞条件培养基中分泌的VEGF对内皮细胞增殖表现出较高的血管生成能力。我们的研究结果表明，VEGF 121主要作为主要的血管生成因子在人成骨细胞的早期阶段。本研究还证实了VEGF 121在成骨细胞发生过程中的差异表达。早期VEGF表达的增加可能是人MSCs诱导成骨的一个有用的标志。
Vascular endothelial growth factor (VEGF)-mediated angiogenesis is essential for bone formation. However, the effect of VEGF on osteoblastic cells during osteoblastogenesis is still controversial. The aim of this,study was to clarify the relationship between osteoblastic cells derived from human mesenchymal stem cells (MSCs). and VEGF -in the early stage of osteoblastic differentiation. Continuous dexamethasone treatment with a low concentration stimulated osteoblastogenesis of MSCs and the expression of VEGF121 mRNA. The VEGF secretion from osteoblastic cells also increased along with osteoblastogenesis. Neuropilin-1, which mainly binds VEGF165, was detected at all stages during early osteoblastogenesis, but VEGF receptor-1 and -2 were not detected on RT-PCR analyses. In this study, VEGF had no direct effect on the proliferation of osteoblastic cells. However, the secreted VEGF in the conditioned medium of osteoblastic cells exhibited high angiogenic power as to endothelial cell proliferation. Our findings indicated that VEGF121 principally acts as the main angiogenic factor in the early stage of human osteoblastogenesis. The present study also demonstrated the differential expression of VEGF121 during osteoblastogenesis. The increase of VEGF in the early stage might be a useful marker of induction of bone formation due to human MSCs.