Identification of Escherichia coli ZapC (YcbW) as a Component of the Division Apparatus That Binds and Bundles FtsZ Polymers
Identification of Escherichia coli ZapC (YcbW) as a Component of the Division Apparatus That Binds and Bundles FtsZ Polymers
复制标题
DOI：
10.1128/jb.01245-10
复制
发表时间：
2011-03-01
影响因子：
3.2
通讯作者：
de Boer, Piet A. J.
中科院分区：
文献类型：
--
作者：
Hale, Cynthia A.;Shiomi, Daisuke;de Boer, Piet A. J.
细菌细胞分裂装置的组装始于在细胞膜的细胞质表面形成Z环。这个过程涉及FtsZ聚合物在中间细胞的积累，以及它们与几个FtsZ结合蛋白的相互作用，这些蛋白共同将聚合物组织成膜相关的环状构型。三种这样的蛋白质，FTSA，ZipA和ZapA，以前已经在大肠杆菌中被鉴定出来。FTSA和ZipA是重要的膜相关分裂蛋白，帮助FtsZ聚合物与内膜连接。ZapA是一种细胞质蛋白质，本身并不是分裂过程所必需的，但有助于其效率，可能是通过促进FtsZ原丝之间的横向相互作用。我们报道了YcbW(ZapC)作为Z环第四个FtsZ结合组分在大肠杆菌中的鉴定。ZapC的结合促进了FtsZ聚合物之间的侧向相互作用，并抑制了FtsZ GTP酶的活性。这一点和更多的证据表明，像ZapA一样，ZapC是一个非必需的Z-环成分，它通过稳定FtsZ的聚合体来促进分裂过程的效率。
Assembly of the cell division apparatus in bacteria starts with formation of the Z ring on the cytoplasmic face of the membrane. This process involves the accumulation of FtsZ polymers at midcell and their interaction with several FtsZ-binding proteins that collectively organize the polymers into a membrane-associated ring-like configuration. Three such proteins, FtsA, ZipA, and ZapA, have previously been identified in Escherichia coli. FtsA and ZipA are essential membrane-associated division proteins that help connect FtsZ polymers with the inner membrane. ZapA is a cytoplasmic protein that is not required for the fission process per se but contributes to its efficiency, likely by promoting lateral interactions between FtsZ protofilaments. We report the identification of YcbW (ZapC) as a fourth FtsZ-binding component of the Z ring in E. coli. Binding of ZapC promotes lateral interactions between FtsZ polymers and suppresses FtsZ GTPase activity. This and additional evidence indicate that, like ZapA, ZapC is a nonessential Z-ring component that contributes to the efficiency of the division process by stabilizing the polymeric form of FtsZ.