AGE-DIFFERENCES IN PHAGOCYTOSIS BY POLYMORPHONUCLEAR LEUKOCYTES MEASURED BY FLOW-CYTOMETRY
AGE-DIFFERENCES IN PHAGOCYTOSIS BY POLYMORPHONUCLEAR LEUKOCYTES MEASURED BY FLOW-CYTOMETRY
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DOI：
10.1002/jlb.39.4.399
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发表时间：
1986-04-01
影响因子：
5.5
通讯作者：
BENDER, BS
中科院分区：
文献类型：
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作者：
NAGEL, JE;HAN, K;BENDER, BS
由于细菌感染，老年人的发病率和死亡率增加。由于多形核白细胞（PMN）是抵抗细菌感染的主要防御，我们利用荧光微球和流细胞术检测了55名中青年（平均年龄41.5岁）和两组老年受试者的PMN吞噬情况：一组35名健康个体（平均年龄74.1岁）生活在家中，第二组11名患有严重精神和身体残疾的居民（平均年龄83.1岁）生活在家庭护理机构。我们测定了吞噬PMN的百分比，每个PMN的微球数量，以及每100个PMN的微球数量。每个吞噬PMN的平均微球数在所有组中相似。中青年组与健康或疾病老年组的吞噬pmn百分比（75.3%比51.5%和43.8%）和每100个吞噬pmn微球数（197.3比131.4和103.2）差异有统计学意义。这些参数在健康和衰弱的老年受试者之间没有显著差异。这些数据文件，有一个年龄相关的增加，代表的PMNs人口有一个缺陷的吞噬能力。
Elderly persons have increased morbidity and mortality due to bacterial infections. Since the polymorphonuclear leukocyte (PMN) is a major defense against bacterial infection, we utilized fluorescent microspheres and flow cytometry to examine phagocytosis by PMNs from 55 young and middle-aged adults (mean age 41.5 yrs) and two groups of elderly subjects: one group of 35 healthy individuals (mean age 74.1 years) living at home, and a second group of 11 residents (mean age 83.1 years) with severe mental and physical disabilities, living in a domiciliary care facility. We determined the percent phagocytic PMNs, the number of microspheres per PMN, and the number of microspheres per 100 PMNs. The mean number of microspheres per phagocytic PMN was similar for all groups. Statistically significant differences were found between the young and middle-aged group and the healthy or ill elderly groups for the percent phagocytic PMNs (75.3% vs 51.5% and 43.8%), and the number of microspheres per 100 phagocytic PMNs (197.3 vs 131.4 and 103.2). There were no significant differences in these parameters between healthy and debilitated elderly subjects. These data document that there is an age-related increase in representation of a population of PMNs which have a defect in phagocytic ability.