The European antibody network's practical guide to finding and validating suitable antibodies for research.
The European antibody network's practical guide to finding and validating suitable antibodies for research.
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DOI：
10.1080/19420862.2015.1100787
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发表时间：
2016
期刊：
影响因子：
5.3
通讯作者：
Banham AH
中科院分区：
文献类型：
--
作者：
Roncador G;Engel P;Maestre L;Anderson AP;Cordell JL;Cragg MS;Šerbec VČ;Jones M;Lisnic VJ;Kremer L;Li D;Koch-Nolte F;Pascual N;Rodríguez-Barbosa JI;Torensma R;Turley H;Pulford K;Banham AH
抗体被广泛用作研究/诊断工具和治疗剂。尽管提供了令人兴奋的研究机会，但众多可用的抗体也提供了令人眼花缭乱的选择。重要的是，并非所有公司都符合最高标准，因此许多试剂无法通过基本的验证测试。令人惊讶的是，抗体是否适合其用途的责任在于它们的使用者。本文浓缩了欧洲单克隆抗体网络的丰富经验，以帮助研究人员识别针对其靶抗原的抗体。提供了一种逐步策略，用于对抗体进行优先排序，并就进一步的基本验证要求做出明智的决定。基于网络的抗体验证指南提供了测试抗体活性和特异性的实用方法。我们的目标是使以前很少或没有抗体表征经验的研究人员能够了解如何确定其抗体对其预期用途的适用性，从而能够以时间和成本效益的方式生成适合出版的高质量抗体数据。
Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.