PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE
PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE
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DOI：
10.1128/jvi.59.2.284-291.1986
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发表时间：
1986-08-01
影响因子：
5.4
通讯作者：
MARTIN, MA
中科院分区：
文献类型：
--
作者：
ADACHI, A;GENDELMAN, HE;MARTIN, MA
我们构建了获得性免疫缺陷综合征相关逆转录病毒的感染性分子克隆。转染后，该克隆除了人T4细胞外，还在多种细胞中指导感染性病毒颗粒的产生。在小鼠、貂、猴和几种人非T细胞系中合成了子代感染性病毒体，表明病毒RNA或蛋白质产生或组装没有任何细胞内障碍。在这些研究过程中，鉴定了对DNA转染极其敏感的人结肠癌细胞系。
We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.