Metabolomic investigations of Ricinus communis for cultivar and provenance determination
Metabolomic investigations of Ricinus communis for cultivar and provenance determination
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DOI：
10.1007/s11306-011-0355-7
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发表时间：
2012-08-01
期刊：
影响因子：
3.6
通讯作者：
Bourne, David J.
中科院分区：
文献类型：
--
作者：
Pigott, Eloise J.;Roberts, Warren;Bourne, David J.
研究了蓖麻6个已知品种的8个样本的代谢组差异，这些差异可用于确定种源和品种。对每个样本三粒种子的七个重复进行H-1 NMR分析，使用多变量统计分析（OPLS-DA）进一步研究收集的数据，根据种源进行分类分离。除了进一步的化学分析的提取物允许鉴定苯丙氨酸，蓖麻碱，蓖麻碱的N-去甲基和O-去甲基类似物，蔗糖作为重要的分子标记物，为特定的品种加载图的分析。为了测试模型的强度，从盲样产生的提取物被用作预测集，并根据种源和品种进行正确分类。
Eight specimens of six known cultivars of Ricinus communis were investigated for differences in their metabolome that could be used to determine both provenance and cultivar. Seven replicates of three seeds per specimen were subjected to H-1 NMR analysis, with the collected data further investigated using multivariate statistical analysis (OPLS-DA), resulting in class separations according to provenance. Analysis of loadings plots in addition to further chemical analysis of the extracts allowed for the identification of phenylalanine, ricinine, the N-demethyl and O-demethyl analogues of ricinine, and sucrose as important molecular markers for particular cultivars. To test the strength of the model, extracts generated from blinded specimens were used as a prediction set and were correctly classified according to provenance and cultivar.