Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydrocortisone
Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydrocortisone
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培养的人二倍体细胞的细胞周期特征：衰老和氢化可的松的影响
DOI：
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发表时间：
1977
影响因子：
5.6
通讯作者：
V. Cristofalo
中科院分区：
文献类型：
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作者：
G. Grove;V. Cristofalo
在正常培养物和通过向培养基中添加氢化可的松延长寿命的培养物中，比较了体外人二倍体细胞寿命的年龄相关变化。对于这两种培养物，随着年龄的增长，活跃增殖池中的细胞比例下降，细胞周期时间的细胞间变异增加。平均细胞周期时间延长老化过程中由于几乎完全在G1期的持续时间的变化。S的持续时间保持恒定，而在接近其寿命结束的晚期传代细胞中观察到G2的小幅延迟。尽管对照和氢化可的松处理的培养物中增殖参数的变化模式相同，但在类固醇存在下，这些变化有所延迟。结果被解释在几个细胞周期模型，并建议控制细胞增殖的事件是敏感的氢化可的松调制在G1和可能的G2期。
Age‐related changes in the cytokinetics of human diploid cells in vitro have been compared in normal cultures and in cultures in which lifespan has been prolonged by the addition of hydrocortisone to the medium. For both cultures, with advancing age the fraction of cells in the actively proliferating pool decreased and the intercellular variation in cell cycle times increased. The average cell cycle time was prolonged during aging due almost entirely to changes in the duration of G1. The duration of S remained constant, while a small delay in G2 was observed in late passage cells near the end of their lifespan. Although the same pattern of change in proliferative parameters occurred in both control and hydrocortisone‐treated cultures, the changes were somewhat delayed in the presence of the steroid. The results are interpreted in terms of several cell cycle models and suggest that the events controlling cell proliferation are sensitive to hydrocortisone modulation during the G1 and possibly the G2 periods.