Microfluidic High-Migratory Cell Collector Suppressing Artifacts Caused by Microstructures
Microfluidic High-Migratory Cell Collector Suppressing Artifacts Caused by Microstructures
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DOI：
10.3390/mi10020116
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发表时间：
2019-02-01
期刊：
影响因子：
3.4
通讯作者：
Omata, Toru
中科院分区：
文献类型：
--
作者：
Ishida, Tadashi;Shimamoto, Takuya;Omata, Toru
细胞群体中少量的高迁移癌细胞使得对高迁移癌细胞的研究变得困难。为了开发这类癌细胞的迁移分析，已经开发了几种微流控装置。然而，他们测量的是受微结构影响的迁移，他们不仅收集高迁移细胞，而且还收集周围的细胞。为了在抑制伪影的同时发现细胞群体中的高迁移细胞，并在最大限度地减少损害的情况下收集这些细胞，我们开发了一种微流控高迁移细胞收集器，能够根据细胞迁移和机械分离来分选癌细胞。当所有细胞都在同一起始线上播种时，高迁移癌细胞从起始线上移动得更远。使用水驱动气球致动器，使用一段细胞粘附面将高迁移细胞分离。使用该细胞收集器，我们选择了在12小时内迁移约100米的高迁移HeLa细胞，并收集了这些细胞。
The small number of high-migratory cancer cells in a cell population make studies on high-migratory cancer cells difficult. For the development of migration assays for such cancer cells, several microfluidic devices have been developed. However, they measure migration that is influenced by microstructures and they collect not only high-migratory cells, but also surrounding cells. In order to find high-migratory cells in cell populations while suppressing artifacts and to collect these cells while minimizing damages, we developed a microfluidic high-migratory cell collector with the ability to sort cancer cells according to cellular migration and mechanical detachment. High-migratory cancer cells travel further from the starting line when all of the cells are seeded on the same starting line. The high-migratory cells are detached using a stretch of cell adhesive surface using a water-driven balloon actuator. Using this cell collector, we selected high-migratory HeLa cells that migrated about 100 m in 12 h and collected the cells.