ROLE OF INTERACTIONS BETWEEN SPOIIAA AND SPOIIAB IN REGULATING CELL-SPECIFIC TRANSCRIPTION FACTOR SIGMA(F) OF BACILLUS-SUBTILIS
ROLE OF INTERACTIONS BETWEEN SPOIIAA AND SPOIIAB IN REGULATING CELL-SPECIFIC TRANSCRIPTION FACTOR SIGMA(F) OF BACILLUS-SUBTILIS
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DOI：
10.1101/gad.8.21.2653
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发表时间：
1994-11-01
影响因子：
10.5
通讯作者：
YUDKIN, MD
中科院分区：
文献类型：
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作者：
DIEDERICH, B;WILKINSON, JF;YUDKIN, MD
遗传实验表明，在孢子形成B.枯草芽孢杆菌，由抗σ因子SpoIIAB和抗抗σ因子SpoIIAA调节。以前，我们报道的生化结果表明，SpoIIAB是一种磷酸激酶，其底物是SpoIIAA和抑制剂的sigma（F）定向转录。我们现在表明，在SpoIIAB和ATP或ADP的存在下，SpoIIAA可以进行两种替代反应。当ATP存在时，SpoIIAA被迅速和完全磷酸化为SpoIIA-磷酸，SpoIIAB立即释放;但在ADP存在下，SpoIIAA与SpoIIAB形成持久的复合物。ADP是ATP磷酸化的抑制剂。此外，我们已经在残基Ser 58处将SpoIIAA突变为天冬氨酸或丙氨酸，所述残基Ser 58是磷酸化的靶。SpoIIAAS 58 D，其明显类似于SpoIIAA-磷酸盐，不能与SpoIIAB形成复合物，并且缺乏抗-抗-σ（F）活性，而SpoIIAAS 58 A，其不能被磷酸化，在ADP或ATP存在下与SpoIIAB形成复合物，并且在体内和体外都具有组成型抗-抗-σ（F）活性。SpoIIAA和SpoIIAB的替代反应（涉及ADP或ATP）可能调节SpoIIAA的抗-抗-sigma能力，从而调节sigma（F）的活性。
Genetic experiments have suggested that sigma(F), the first compartment-specific transcription factor in sporulating B. subtilis, is regulated by an anti-sigma factor SpoIIAB and an anti-anti-sigma factor SpoIIAA. Previously, we reported biochemical results demonstrating that SpoIIAB is both a phosphokinase whose substrate is SpoIIAA and an inhibitor of sigma(F)-directed transcription. We now show that in the presence of SpoIIAB and ATP or ADP, SpoIIAA can undergo two alternative reactions. When ATP is present, SpoIIAA is phosphorylated rapidly and completely to SpoIIA-phosphate, and SpoIIAB is immediately released; but in the presence of ADP, SpoIIAA forms a long lasting complex with SpoIIAB. ADP is an inhibitor of the phosphorylation by ATP. Furthermore, we have mutated SpoIIAA at residue Ser 58, the target for phosphorylation, to aspartate or alanine. SpoIIAAS58D, which apparently resembles SpoIIAA-phosphate, is unable to make a complex with SpoIIAB and is devoid of anti-anti-sigma(F) activity, whereas SpoIIAAS58A, which cannot be phosphorylated, makes complexes with SpoIIAB in the presence of ADP or ATP and has constitutive anti-anti-sigma(F) activity both in vivo and in vitro. It seems likely that the alternative reactions of SpoIIAA and SpoIIAB, involving ADP or ATP, regulate the anti-anti-sigma capacity of SpoIIAA and hence the activity of sigma(F).