Sugar transport by renal plasma membrane vesicles
Sugar transport by renal plasma membrane vesicles
复制标题
肾质膜囊泡的糖转运
DOI：
10.1007/bf01941077
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
G. Sachs
中科院分区：
文献类型：
--
作者：
R. Kinne;H. Murer;E. Kinne;M. Thees;G. Sachs
概述在来自刷状缘和基底侧膜的囊泡上进行d-和l-葡萄糖的摄取研究。糖的摄取进入囊泡是敏感的，不依赖于葡萄糖代谢。在刷状缘囊泡中，葡萄糖转运是Na+依赖性的，根皮苷可抑制葡萄糖转运，在初始Na+梯度存在下，囊泡/培养基比值>1。基底-侧膜吸收up-glucose的速度比l-glucose快，但吸收d-glucose对钠去除的敏感性显著降低，与刷状缘部分相比，根皮苷仅中度抑制。
SummaryUptake studies ofd- andl-glucose were performed on vesicles derived from brush-border and basal-lateral membranes. The uptake of the sugars into the vesicles was osmotically sensitive and independent of glucose metabolism. In brush-border vesiclesd-glucose but notl-glucose transport was Na+-dependent, was inhibited by phlorizin, and showed a transitory vesicle/medium ratio >1, in the presence of an initial Na+ gradient. Basal-lateral membranes take upd-glucose faster thanl-glucose, but thed-glucose uptake is significantly less sensitive to sodium removal and only moderately inhibited by phlorizin as compared to the brush-border fraction.