Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter
Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter
复制标题
通过用 Pspac 启动子替换天然启动子来增强枯草芽孢杆菌 fmbR 的表面活性素产量
DOI：
10.1139/w09-044
复制
发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Lu, Zhaoxin
中科院分区：
文献类型：
--
作者：
Sun, Huigang;Bie, Xiaomei;Lu, Zhaoxin
枯草芽孢杆菌fmbR-1从野生型B获得。通过用诱导型启动子Pspac替换表面活性素操纵子的天然启动子，将枯草芽孢杆菌fmbR转化为表达载体。重组B. B.枯草杆菌fmbR-1产生更多的抗菌物质比野生型菌株。高产表型与对蜡样芽孢杆菌的拮抗活性增强有关。重组fmbR-1的表面活性素的HPLC峰显示出与野生型菌株相似的脂肽模式，并且重组fmbR-1的表面活性素产量是野生型菌株的约5倍，而没有异丙基β-D-1-硫代半乳糖苷的诱导。然而，当被异丙基β-D-1-硫代半乳糖苷诱导时，表面活性素的产量比野生型菌株增加约10倍。
Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enhancement of antagonistic activities against Bacillus cereus. HPLC peaks of surfactin for recombinant fmbR-1 showed patterns of lipopeptides similar to those of the wild-type strain, and surfactin production of the recombinant fmbR-1 was up to about fivefold greater than that of the wild-type strain without induction by isopropyl beta-D-1-thiogalactopyranoside. However, the production of surfactin increased to about 10-fold more than that of the wild-type strain when it was induced by isopropyl beta-D-1-thiogalactopyranoside.