Autoradiographic localization of peripheral benzodiazepine, dihydroalprenolol and arginine vasopressin binding sites in the pituitaries of control, stalk transected and Brattleboro rats.
Autoradiographic localization of peripheral benzodiazepine, dihydroalprenolol and arginine vasopressin binding sites in the pituitaries of control, stalk transected and Brattleboro rats.
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对照大鼠、茎横断大鼠和布拉特尔伯勒大鼠垂体中外周苯二氮卓类、二氢阿普洛尔和精氨酸加压素结合位点的放射自显影定位。
DOI：
10.1159/000124625
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发表时间：
1986
影响因子：
4.1
通讯作者：
G. Wilkin
中科院分区：
文献类型：
--
作者：
S. Bunn;Michael R. Hartley;G. Wilkin
在垂体柄横断前后，检查大鼠垂体中[3H]精氨酸加压素、[3H]螺哌隆、[3H]GABA、[3H]二氢阿普洛尔和外周型苯二氮卓配体[3H]Ro5-4864的放射自显影分布。茎横断使神经部的细胞结构发生了巨大的变化。神经胶质纤维酸性蛋白（垂体细胞中报道的星形胶质细胞标记物）在柄横断后增加，而神经丝（神经元神经支配的标记物）丢失。这些结构变化证明了茎横切的成功，从而可以解释三个叶上几种[3H]-配体的密度变化。 [3H]Ro5-4864结合显着增加，表明该位点位于垂体细胞上。相反，神经部上的[3H]螺哌酮和[3H]精氨酸加压素结合密度降低。在缺乏垂体加压素的突变型尿崩症大鼠（Brattleboro）中，神经部检测不到[3H]精氨酸加压素结合。 [3H]二氢阿普洛尔和[3H]GABA结合位点未因病变而改变。这些结果根据垂体细胞上功能性受体的出现及其在神经水体分泌中的可能作用进行了讨论。
The autoradiographic distribution of [3H]arginine vasopressin, [3H]spiperone, [3H]GABA, [3H]dihydroalprenolol and the peripheral-type benzodiazepine ligand [3H]Ro5-4864 were examined in the rat pituitary before and after pituitary stalk transection. Stalk transection produced dramatic changes in the cellular architecture of the pars nervosa. Glial fibrillary acidic protein, an astrocyte marker reported in pituicytes, increased after stalk transection, whereas neurofilament, a marker for neuronal innervation, was lost. These structural changes demonstrated a successful stalk transection, permitting interpretation of changes in the densities of several [3H]-ligands over the three lobes. [3H]Ro5-4864 binding was markedly increased, suggesting that this site was located on the pituicytes. Conversely [3H]spiperone and [3H]arginine vasopressin binding density over the pars nervosa decreased. In the mutant diabetes insipidus rat (Brattleboro), which lacks pituitary vasopressin, [3H]arginine vasopressin binding was undetectable in the pars nervosa. [3H]dihydroalprenolol and [3H]GABA binding sites were unchanged by the lesion. These results are discussed in terms of the occurrence of functional acceptors on pituicytes and their possible role in neurohydrophyseal secretions.