Microbiota modulation and effects on metabolic biomarkers by orange juice: a controlled clinical trial
Microbiota modulation and effects on metabolic biomarkers by orange juice: a controlled clinical trial
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DOI：
10.1039/c9fo02623a
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发表时间：
2020-02-01
期刊：
影响因子：
6.1
通讯作者：
Cesar, Thais
中科院分区：
文献类型：
--
作者：
Fidelix, Melaine;Milenkovic, Dragan;Cesar, Thais
在一项对照临床试验中研究了习惯性橙子汁消费对微生物群、脂质和糖代谢的影响。临床程序如下：10名妇女有一个正常的饮食没有橙子汁30天（OJ-免费饮食），然后是一个正常的饮食加300毫升d（-1）橙子汁60天（OJ-饮食），30天的正常饮食没有橙子汁（洗脱）。监测生化和饮食参数，并每30天采集一次血液、尿液和粪便样本，直至研究结束。尿中橙皮苷和柚皮苷代谢产物通过UHPLC鉴定，粪便微生物群组成通过16 S rRNA测定。在OJ饮食结束时，葡萄糖（-6.5%）、胰岛素（-33%）、胰岛素抵抗（-44%）、LDL-C（-16%）和甘油三酯（-30%）降低。洗脱后，这些参数恢复到其初始值。在实验期间，体重或脂肪无变化。肠道细菌，乳杆菌属，阿克曼氏菌属，和瘤胃球菌属，橙子汁干预后增加。此外，这些细菌与胰岛素、HOMA-IR、甘油三酯、总胆固醇和LDL-C呈负相关，但与HDL-C呈正相关。总之，橙子汁显示出益生元效应，调节肠道微生物群，同时改善脂肪和脂质分布。
The impact of habitual orange juice consumption on microbiota, lipid and sugar metabolism was investigated in a controlled clinical trial. The clinical procedure is as follows: ten women who had a regular diet without orange juice for 30 days (OJ-free diet), followed by a regular diet plus 300 ml d(-1) orange juice for 60 days (OJ-Diet), and 30 days with a regular diet without orange juice (Washout). Biochemical and dietary parameters were monitored, and blood, urine and stool samples were collected every 30 days until the end of the study. Hesperidin and naringin metabolites in the urine were identified by UHPLC, and the microbiota composition of the feces was determined by 16S rRNA. At the end of the OJ-Diet, there was a reduction in glucose (-6.5%), insulin (-33%), insulin resistance (-44%), LDL-C (-16%) and triglycerides (-30%). After the washout, these parameters returned to their initial values. There were no changes in the body weight or fat during the experimental time. The intestinal bacteria, Lactobacillus spp., Akkermansia spp., and Ruminococcus spp., increased after the intervention with orange juice. In addition, an inverse correlation was detected between these bacteria and glycemia, insulin, HOMA-IR, triglycerides, total cholesterol and LDL-C, but a direct correlation with HDL-C. In conclusion, orange juice showed a prebiotic effect, modulating the intestinal microbiota while improving the glycemia and lipid profiles.