5'-TERMINAL 7-METHYLGUANOSINE IN EUKARYOTIC MESSENGER-RNA IS REQUIRED FOR TRANSLATION
5'-TERMINAL 7-METHYLGUANOSINE IN EUKARYOTIC MESSENGER-RNA IS REQUIRED FOR TRANSLATION
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DOI：
10.1038/255033a0
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发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
SHATKIN, AJ
中科院分区：
文献类型：
--
作者：
MUTHUKRISHNAN, S;BOTH, GW;SHATKIN, AJ
未甲基化的呼肠孤病毒和VSV mRNA通过从小麦胚芽和小鼠L细胞制备的蛋白质合成提取物特异性甲基化以形成m7 G（5′）ppp（5′）N类型的5′-末端结构。网织红细胞mRNA的5′端也含有m7 G。具有5′-末端m7 G的mRNA在体外刺激蛋白质合成。通过β-消除去除m7 G消除了mRNA的翻译。
Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5′-terminal structures of the type, m7G (5′)ppp (5′) N by protein synthesizing extracts prepared from wheat germ and mouse L cells. Reticulocyte mRNA also contains 5′-terminal m7G. mRNAs having 5′-terminal m7G stimulate protein synthesis in vitro. Removal of m7G by β-elimination abolishes translation of the mRNAs.