Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics.
Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics.
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钙调蛋白与COPI蛋白Sec28和CoPII蛋白Sec13的关联，通过定量蛋白质组学揭示。
DOI：
10.1371/journal.pone.0025280
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Heitman J
中科院分区：
文献类型：
--
作者：
Kozubowski L;Thompson JW;Cardenas ME;Moseley MA;Heitman J
Calcineurin is a calcium-calmodulin-dependent serine/threonine specific protein phosphatase operating in key cellular processes governing responses to extracellular cues. Calcineurin is essential for growth at high temperature and virulence of the human fungal pathogen Cryptococcus neoformans but the underlying mechanism is unknown. We performed a mass spectrometry analysis to identify proteins that associate with the calcineurin A catalytic subunit (Cna1) in C. neoformans cells grown under non-stress and high temperature stress conditions. A novel prioritization strategy for mass spectrometry data from immunoprecipitation experiments identified putative substrates and proteins potentially operating with calcineurin in common pathways. Cna1 co-purified with proteins involved in membrane trafficking including the COPI component Sec28 and the COPII component Sec13. The association of Cna1 with Sec28 and Sec13 was confirmed by co-immunoprecipitation. Cna1 exhibited a dramatic change in subcellular localization during high temperature stress from diffuse cytoplasmic to ER-associated puncta and the mother-bud neck and co-localized with Sec28 and Sec13.
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影响因子：
11.4
作者：
Görlach, J;Fox, DS;Heitman, J
通讯作者：
Heitman, J
影响因子：
3.3
作者：
Conboy, MJ;Cyert, MS
通讯作者：
Cyert, MS
影响因子：
64.8
作者：
Andersen, JS;Wilkinson, CJ;Mann, M
通讯作者：
Mann, M
DOI：
10.1083/jcb.135.1.19
发表时间：
1996-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bannykh SI;Rowe T;Balch WE
通讯作者：
Balch WE
影响因子：
11.4
作者：
Duden, R;Kajikawa, L;Schekman, R
通讯作者：
Schekman, R