Transcriptome of 3D7 and its gametocyte-less derivative F12 Plasmodium falciparum clones during erythrocytic development using a gene-specific microarray assigned to gene regulation, cell cycle and transcription factors
Transcriptome of 3D7 and its gametocyte-less derivative F12 Plasmodium falciparum clones during erythrocytic development using a gene-specific microarray assigned to gene regulation, cell cycle and transcription factors
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DOI：
10.1016/j.gene.2004.07.004
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发表时间：
2004-10-27
期刊：
影响因子：
3.5
通讯作者：
Vaquero, C
中科院分区：
文献类型：
--
作者：
Gissot, M;Refour, P;Vaquero, C
在恶性疟原虫复杂的生命周期中，通过蚊子和人类，红细胞周期负责疟疾的疾病和传播。对寄生虫发育过程中发生的事件的调控，如增殖和分化，意味着对转录活动的精细控制，而转录活动反过来又控制着一组基因的表达谱。尽管激酶和转录因子被认为在这一事件中起着关键作用，但对支持配子体承诺的途径仍知之甚少。为了了解可能参与早期配子体发生的基因表达谱变化的分子机制，比较了两个克隆3D7及其无配子体衍生物F12在红细胞无性发育的5个时间点的转录组。我们使用了一个包含150个PCR片段的主题DNA微阵列，这些片段代表了恶性疟原虫参与信号转导、细胞周期和转录调控的基因。我们鉴定了几个引起不同表达谱的基因，其中一些与基因调控有关或编码可能的转录因子。在两个克隆中观察到的转录因子和激酶转录本的差异表达可能启发了可能在F12克隆的早期配子体发生中起作用的基因。(C)2004爱思唯尔B.V.保留所有权利。
During the complex life cycle of Plasmodium falciparum, through mosquito and human, the erythrocytic cycle is responsible for malarial disease and transmission. The regulation of events that occur during parasite development, such as proliferation and differentiation, implies a fine control of transcriptional activities that in turn governs the expression profiles of sets of genes. Pathways that underline gametocyte commitment are yet poorly understood even though kinases and transcription factors have been assumed to play a crucial role in this event. In order to understand the molecular mechanisms controlling the variation of gene expression profiles that might participate in early gametocytogenesis, the transcriptome of two clones, 3D7 and its gametocyte-less derivative F 12, was compared at five time points of the erythrocytic asexual development. We have used a thematic DNA microarray containing 150 PCR fragments, representative of P. falcipaparum genes involved in signal transduction, cell cycle and transcriptional regulation. We identified several genes eliciting different expression profiles among which some implicated in gene regulation or encoding putative transcription factors. The differential expression of transcription factor and kinase transcripts observed in the two clones may enlighten genes that might have a role in impairment of the early gametocytogenesis of the F12 clone. (C) 2004 Elsevier B.V. All rights reserved.