ROCR, A NOVEL REGULATORY PROTEIN CONTROLLING ARGININE UTILIZATION IN BACILLUS-SUBTILIS, BELONGS TO THE NTRC/NIFA FAMILY OF TRANSCRIPTIONAL ACTIVATORS
ROCR, A NOVEL REGULATORY PROTEIN CONTROLLING ARGININE UTILIZATION IN BACILLUS-SUBTILIS, BELONGS TO THE NTRC/NIFA FAMILY OF TRANSCRIPTIONAL ACTIVATORS
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DOI：
10.1128/jb.176.5.1234-1241.1994
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发表时间：
1994-03-01
影响因子：
3.2
通讯作者：
DEBARBOUILLE, M
中科院分区：
文献类型：
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作者：
CALOGERO, S;GARDAN, R;DEBARBOUILLE, M
枯草芽孢杆菌可以利用铵和多种氨基酸作为唯一的氮源。精氨酸或鸟氨酸的利用在枯草芽孢杆菌的 sigma L 缺陷菌株中被消除，表明参与该途径的一个或多个基因是由 sigma L-RNA 聚合酶全酶转录的。三个枯草芽孢杆菌基因，称为 rocA、rocB 和 rocC，似乎形成操纵子，仅在 sacTPA 位点附近发现（P. Glaser、F. Kunst、M. Arnaud、M.-P. Coudart、W. Gonzales、M.-F. Hullo、M. Ionescu、B. Lubochinsky、L. Marcelino、I. Moszer、E.普雷塞坎，M.桑塔纳，E. 施奈德 (J. Schweizer)、A. Vertes、G. Rapoport 和 A. Danchin (Mol)。微生物。 10：371-384，1993）。该推定操纵子的表达由精氨酸诱导，并且是 σ L 依赖性的。获得了rocA转录受损的突变体。其中一个突变体被用作受体，克隆并测序了一个名为 rocR 的新调控基因。该基因编码 52 kDa 的多肽，属于 NtrC/NifA 转录激活因子家族。在 rocABC 基因的上游也鉴定出了与大肠杆菌中 NtrC 高度相似的上游激活序列。含有 rocR 无效突变的枯草芽孢杆菌菌株无法使用精氨酸作为唯一的氮源，表明 RocR 是精氨酸分解代谢的正调节因子。继 LevR 之后，RocR 是革兰氏阳性菌中刺激 sigma 54 依赖性启动子的激活剂的第二个例子。
Bacillus subtilis can use ammonium and various amino acids as sole nitrogen sources. The utilization of arginine or ornithine is abolished in a sigma L-deficient strain of B. subtilis, indicating that one or several genes involved in this pathway are transcribed by a sigma L-RNA polymerase holoenzyme. Three B. subtilis genes, called rocA, rocB, and rocC, which seem to form an operon, mere found near the sacTPA locus (P. Glaser, F. Kunst, M. Arnaud, M.-P. Coudart, W. Gonzales, M.-F. Hullo, M. Ionescu, B. Lubochinsky, L. Marcelino, I. Moszer, E. Presecan, M. Santana, E. Schneider, J. Schweizer, A. Vertes, G. Rapoport, and A. Danchin, Mol. Microbiol. 10:371-384, 1993). The expression of this putative operon is induced by arginine and is sigma L dependent. Mutants impaired in the transcription of rocA were obtained. One of these mutants was used as recipient to clone and sequence a new regulatory gene, called rocR. This gene encodes a polypeptide of 52 kDa which belongs to the NtrC/NifA family of transcriptional activators. Upstream activating sequences highly similar to those of NtrC in Escherichia coli were also identified upstream from the rocABC genes. A B. subtilis strain containing a rocR null mutation is unable to use arginine as the sole nitrogen source, indicating that RocR is a positive regulator of arginine catabolism. After LevR, RocR is the second example of an activator stimulating sigma 54-dependent promoters in gram-positive bacteria.