Cell fate specification during calvarial bone and suture development
Cell fate specification during calvarial bone and suture development
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DOI：
10.1016/j.ydbio.2007.08.028
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发表时间：
2007-11-15
影响因子：
2.7
通讯作者：
Rice, David P. C.
中科院分区：
文献类型：
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作者：
Lana-Elola, Eva;Rice, Ritva;Rice, David P. C.
在这项研究中，我们解决了什么细胞机制控制颅骨生长的基本问题，相反，当颅骨接近形成缝合线时，缝合间充质细胞的命运是什么。有证据表明，骨祖细胞群的大小决定了颅骨骨生长的速度。在颅骨培养中，我们减少了骨祖细胞的增殖；然而，我们没有观察到同样程度的顶骨生长减少。这种差异促使我们研究缝线间充质细胞是否参与顶骨的生长。我们发现靠近顶骨成骨前缘的间充质细胞可以分化成成骨细胞谱系，并可以并入生长的骨中。相反，缝线中间的间充质细胞没有并入骨中，保持未分化状态。因此，间充质细胞的命运取决于它们在缝合线内的位置。在本研究中，我们发现成骨前沿骨祖细胞的持续增殖是颅骨骨生长的主要机制，但重要的是，我们发现缝合间充质细胞可以促进颅骨骨生长。这些发现有助于我们了解膜内骨化的一般机制，膜内骨化不仅发生在颅骨和面部骨骼发育期间，也发生在大多数骨骼的表面骨膜中。(c) 2007爱思唯尔公司版权所有。
In this study we have addressed the fundamental question of what cellular mechanisms control the growth of the calvarial bones and conversely, what is the fate of the sutural mesenchymal cells when calvarial bones approximate to form a suture. There is evidence that the size of the osteoprogenitor cell population determines the rate of calvarial bone growth. In calvarial cultures we reduced osteoprogenitor cell proliferation; however, we did not observe a reduction in the growth of parietal bone to the same degree. This discrepancy prompted us to study whether suture mesenchymal cells participate in the growth of the parietal bones. We found that mesenchymal cells adjacent to the osteogenic fronts of the parietal bones could differentiate towards the osteoblastic lineage and could become incorporated into the growing bone. Conversely, mid-suture mesenchymal cells did not become incorporated into the bone and remained undifferentiated. Thus mesenchymal cells have different fate depending on their position within the suture. In this study we show that continued proliferation of osteoprogenitors in the osteogenic fronts is the main mechanism for calvarial bone growth, but importantly, we show that suture mesenchyme cells can contribute to calvarial bone growth. These findings help us understand the mechanisms of intramembranous ossification in general, which occurs not only during cranial and facial bone development but also in the surface periosteum of most bones during modeling and remodeling. (c) 2007 Elsevier Inc. All rights reserved.