Three pheromone-binding proteins help segregation between two Helicoverpa species utilizing the same pheromone components
Three pheromone-binding proteins help segregation between two Helicoverpa species utilizing the same pheromone components
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三种信息素结合蛋白利用相同的信息素成分帮助两个 Helicoverpa 物种之间的分离
DOI：
10.1016/j.ibmb.2012.06.004
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发表时间：
2012-09-01
影响因子：
3.8
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
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作者：
Guo, Flao;Huang, Ling-Qiao;Wang, Chen-Zhu
两个兄弟物种棉铃虫和棉铃虫利用相同的两种醛作为性信息素，但比例相反。在这两个物种中，三种气味结合蛋白（OBP）可归类为信息素结合蛋白（PBP）。为了研究这三种 PBP 在两性之间化学通讯中的作用及其作用模式，我们在细菌中表达了这些蛋白质，并制备了缺乏 C 末端区域的突变体。使用多克隆抗体，我们发现这三种PBPs的表达基本上局限于两性和两物种的触角。荧光探针 N-苯基-1-萘胺在整个 pH 范围内的结合实验表明，野生型蛋白质的亲和力在低 pH 下降低，而突变体的亲和力不受影响或影响较小，这表明这些蛋白质中发生了 C 末端的构象变化，正如其他鳞翅目 OBP 所报道的那样。所有三种蛋白质以相似的强度结合信息素成分及其相应的醇和乙酸酯。然而，它们对不同长度的直链醇和醛表现出显着的选择性，对 PBP1 的 13-15 个碳原子和 PBP2 的 12-14 个碳原子的配体具有最佳的亲和力。我们建议所有三种 PBP 可能合作构建独特的嗅觉图像，这有助于避免两个物种之间以及与其他夜行动物之间的交叉交配。 (C) 2012 Elsevier Ltd. 保留所有权利。
The two sibling species Helicovezpa armigera and Helicoverpa assulta utilise the same two aldehydes as their sex pheromones, but in opposite ratios. In both species three odorant-binding proteins (OBPs) can be classified as pheromone-binding proteins (PBPs). To investigate the role of these three PBPs in chemical communication between sexes and their mode of action, we have expressed the proteins in bacteria and prepared mutants lacking their C-terminal regions. Using polyclonal antibodies we found that the expression of the three PBPs is basically confined to the antennae of both sexes and both species. Binding experiments with the fluorescent probe N-phenyl-1-naphthylamine across a pH range indicated that, the affinity of wild-type proteins decreases at low pH, while that of the mutants is not or less affected, suggesting that a conformational change of the C-terminus occurs in these proteins, as reported for other lepidopteran OBPs. All three proteins bind with similar strength both pheromone components, as well as their corresponding alcohols and acetates. However, they exhibit significant selectivity to linear alcohols and aldehydes of different length, with optimal affinities to the ligand of 13-15 carbon atoms for PBP1 and 12-14 carbon atoms for PBP2. We suggest that all three PBPs might cooperate to build a unique olfactory image, that could help avoiding cross-mating between the two species and with other noctuids. (C) 2012 Elsevier Ltd. All rights reserved.