Isolates of Burkholderia pseudomallei from northern Australia are distinct by multilocus sequence typing, but strain types do not correlate with clinical presentation
Isolates of Burkholderia pseudomallei from northern Australia are distinct by multilocus sequence typing, but strain types do not correlate with clinical presentation
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DOI：
10.1128/jcm.42.12.5477-5483.2004
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发表时间：
2004-12-01
影响因子：
9.4
通讯作者：
Currie, BJ
中科院分区：
文献类型：
--
作者：
Cheng, AC;Godoy, D;Currie, BJ
类鼻疽病是由寄生虫类鼻疽伯克霍尔德氏菌引起的疾病。以往的研究表明，一些菌株的嗜性和不同的毒力。在这项研究中，我们定义菌株的多位点序列分型（MLST）的分离株从澳大利亚的北领地的顶端，并与世界各地的其他菌株分型的结果进行比较。我们专门寻找菌株类型的临床和地理相关性。在87个澳大利亚分离物中，确定了48个序列类型。本研究中的序列类型在世界其他地方都没有发现。菌株广泛分布在整个地区，疾病的不同表现，包括神经和前列腺感染，与许多不同的菌株。脉冲场凝胶电泳和MLST之间有很好的一致性，两种分型方法具有相似的菌株区分水平。这项工作表明，宿主和环境因素可能比感染菌株类型在确定疾病表现方面更重要。这项研究中发现的独特但多样的菌株类型可能反映了澳大利亚数百万年来的地理隔离。
Melioidosis is the disease caused by the saprophytic organism Burkholderia pseudomallei. Previous studies have suggested some strain tropism and differential virulence. In this study, we defined strains by multilocus sequence typing (MLST) of isolates taken from the Top End of Australia's Northern Territory and compared the results with those of other strains typed worldwide. We specifically sought clinical and geographical correlates of strain types. Among 87 Australian isolates, 48 sequence types were defined. None of the sequence types in this study has been found elsewhere in the world. Strains were distributed widely throughout the region, and the different presentations of disease, including neurological and prostatic infection, were associated with many different strains. There was excellent congruence between pulsed-field gel electrophoresis and MLST, and the two typing methods had a similar level of strain discrimination. The work suggests that host and environmental factors may be more important in determining disease presentation than infecting strain type. It is possible that the distinct but diverse strain types found in this study reflect Australia's geographical isolation over many millions of years.