The separation of acetic bacteria advantage lies in acid production and the grey relational analysis on its proliferation and acid production with ethyl alcohol
The separation of acetic bacteria advantage lies in acid production and the grey relational analysis on its proliferation and acid production with ethyl alcohol
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发表时间：
2015
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通讯作者：
Ye Ri-yin
中科院分区：
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作者：
Ye Ri-yin
为了从自然界中筛选出具有产酸优势的醋酸菌，明确其产酸量、细胞增殖与发酵底物乙醇浓度的关系。本试验以腐烂的菠萝为原料，对醋酸菌进行分离纯化，筛选出可溶性钙环较大的菌落，进行发酵产酸。通过测定发酵液的含酸量，找出产酸量最高的菌株。并进行了一系列酒精浓度的恒温发酵试验。一段时间后检测细胞增殖和产酸。采用灰色关联分析法对这些数据进行分析，通过计算得到了酒精浓度与产酸量和细胞增殖的两个关联度。并对它们的关系序列进行了比较。筛选出一株产酸能力较强的醋酸菌S1，其产酸量、细胞增殖与酒精浓度的相关度分别为0.670和0.517。结果表明，S1的产酸量与酒精浓度之间存在显著的相关性（关联度为0。细胞增殖与酒精浓度无显著相关性（相关度0. 6）。乙醇对产酸的影响大于对细胞增殖的影响。
In order to get acetic bacterial strain which had advantage lays in acid production from nature,clear and definite the relationship between its acid production,cell proliferation and ethanol concentration of fermentation substrate. In the experiment acetic bacteria was separation and purification with rotten pineapple,the bacterial colony which had bigger soluble calcium circle was selected and put into fermentation for acid production. Then the strain which had the highest acid prodution was found out after determined acid content of fermentation broth. It was put into the fermentation experiment on constant temperature with a series of alcohol concentration. After a time the cell proliferation and acid production were detected. Datas of these was put into analysis based on the grey relational analysis, by calculation two relevancies between alcohol concentration and acid production and cell proliferation were acquired. And their relational sequence was put into compare. As the result,a acetic bacterial strain S1 which had advantage lies in acid production was got out,the relational degree between acid production,cell proliferation of S1 and alcohol concentration respectively was 0.670 and 0.517. The results showed that the correlation was outstanding between acid production of S1 and alcohol concentration( relational degree 0. 6), and the correlation was non- significant between cell proliferation and alcohol concentration(relational degree0.6). The effect of ethyl alcohol for acid production was greater than that for cell proliferation.