Stimulation of glutamate receptor protein synthesis and membrane insertion within isolated neuronal dendrites
Stimulation of glutamate receptor protein synthesis and membrane insertion within isolated neuronal dendrites
复制标题
DOI：
10.1073/pnas.97.21.11545
复制
发表时间：
2000-10-10
影响因子：
11.1
通讯作者：
Eberwine, J
中科院分区：
文献类型：
--
作者：
Kacharmina, JE;Job, C;Eberwine, J
mrna在树突上的选择性亚细胞定位和最近对局部蛋白合成的证明，突出了突触后位点在细胞间通讯调节中的潜在作用。我们发现，表位标记的谷氨酸受体2亚基GluR2 mRNA转染到离体海马神经元树突后，在药物刺激下被翻译。此外，对N端标记GluR2的共聚焦成像显示，新合成的GluR2蛋白可以整合到树突膜中，N端在树突外定位，这些数据表明，整合膜蛋白可以在树突中合成，并且可以局部整合到细胞膜中。
The selective subcellular localization of mRNAs to dendrites and the recent demonstration of local protein synthesis have highlighted the potential role of postsynaptic sites in modulation of cell-cell communication. We show that epitope-tagged subunit 2 of the ionotopic glutamate receptor, GluR2, mRNA transfected into isolated hippocampal neuronal dendrites is translated in response to pharmacologic stimulation. Further, confocal imaging of N-terminally labeled GluR2 reveals that the newly synthesized GluR2 protein can integrate into the dendritic membrane with the N terminus externally localized, These data demonstrate that integral membrane proteins can be synthesized in dendrites and can locally integrate into the cell membrane.