Production of laccase, manganese peroxidase and lignin peroxidase by Brazilian marine-derived fungi
Production of laccase, manganese peroxidase and lignin peroxidase by Brazilian marine-derived fungi
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DOI：
10.1016/j.enzmictec.2009.07.014
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发表时间：
2010-01-07
影响因子：
3.4
通讯作者：
Sette, Lara Duraes
中科院分区：
文献类型：
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作者：
Bonugli-Santos, Rafaella Costa;Durrant, Lucia Regina;Sette, Lara Duraes
海洋真菌是寻找具有相关特征的新化合物的潜在来源。其中，木质素分解酶在许多领域具有潜在的应用，包括环境和工业部门。本工作旨在采用统计实验设计，评价三种海洋来源真菌（核曲霉CBMAI 849、枝孢枝霉菌CBMAI 857和总状毛霉CBMAI 847）在不同碳源和盐度条件下的酶活性。当这些真菌在麦芽提取物中培养时，检测到MnP、LiP和漆酶，然而当在含有葡萄糖和麦麸的基础培养基上生长时，未检测到LiP，但观察到MnP和漆酶的增加。通过表面响应进行统计分析，结果显示高值的锰过氧化物酶和漆酶活性下12.5%和23%（w/v）的盐度，突出了这些真菌的潜在用途，工业应用和污染的网站具有高盐浓度的生物修复。其中M. L racemosus CBMAI 847的木质素降解酶，这是首次报道该属的放线菌产生木质素降解酶。(C)2009 Elsevier Inc. All rights reserved.
Marine-derived fungi are a potential for the search of new compounds with relevant features. Among these, the ligninolytic enzymes have potential applications in a large number of fields, including the environmental and industrial sectors. This is the work aimed to evaluate the enzymatic activities of three marine-derived fungi (Aspergillus sclerotiorum CBMAI 849, Cladosporium cladosporioides CBMAI 857 and Mucor racemosus CBMAI 847) under different carbon sources and salinity conditions by using statistical experimental design. MnP, LiP and laccase were detected when these fungi were cultured in malt extract, however when grown on basal medium containing glucose and wheat bran LiP was not detected and yet an increase in MnP and laccase was observed. Statistical analysis through surface responses was performed and results showed high values of MnP and laccase activities under 12.5% and 23% (w/v) salinity, highlighting the potential use of these fungi for industrial applications and in bioremediation of contaminated sites having high salt concentrations. The highest values for Lip (75376.34 UI L-1), MnP (4484.30 IU L-1) and laccase (898.15 UI L-1) were obtained with the fungus M. racemosus CBMAI 847 and it is the first report concerning ligninolytic enzymes production by a zygomycete from this genus. (C) 2009 Elsevier Inc. All rights reserved.