Drosophila Rac1 controls motor axon guidance.
Drosophila Rac1 controls motor axon guidance.
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DOI：
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发表时间：
1998-02
期刊：
影响因子：
4.6
通讯作者：
Nancy Kaufmann;Zachary P. Wills;D. V. Vactor
中科院分区：
文献类型：
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作者：
Nancy Kaufmann;Zachary P. Wills;D. V. Vactor
先前对节间神经b发育的遗传研究已经确定了几种细胞表面蛋白，这些蛋白是正确的轴突引导到适当的目标肌肉所必需的。我们提供的证据表明，小的GTPase Drac1在这一引导过程中也起着关键作用。显性负突变drak1 （N17）的神经元表达导致轴突绕过并延伸到正常突触伙伴之外。这种表型通过药物阻断肌动蛋白组装而持续复制。drak1 （N17）与受体酪氨酸磷酸酶Dlar之间的遗传相互作用表明，节段间神经b的引导需要多个收敛信号的整合。
Previous genetic studies of intersegmental nerve b development have identified several cell-surface proteins required for correct axon guidance to appropriate target muscles. Here we provide evidence that the small GTPase Drac1 also plays a key role in this guidance process. Neuronal expression of the dominant negative mutation Drac1(N17) causes axons to bypass and extend beyond normal synaptic partners. This phenotype is consistently reproduced by pharmacological blockade of actin assembly. Genetic interactions between Drac1(N17) and the receptor-tyrosine phosphatase Dlar suggest that intersegmental nerve b guidance requires the integration of multiple, convergent signals.