Capillary electrophoresis as a tool for optimization of multiplex PCR reactions
Capillary electrophoresis as a tool for optimization of multiplex PCR reactions
复制标题
DOI：
10.1007/s002160000641
复制
发表时间：
2001-02-01
期刊：
影响因子：
--
通讯作者：
Vallone, PM
中科院分区：
文献类型：
--
作者：
Butler, JM;Ruitberg, CM;Vallone, PM
今天，复制DNA分子的多个区域是常规使用聚合酶链式反应(PCR)进行的，这一过程通常被称为多重PCR。多重PCR反应的发展包括设计引物组和检查这些引物组的各种组合和不同的反应成分和/或热循环条件，优化多重PCR反应以获得平衡的一组扩增片段的过程可能是耗时和劳动密集型的。毛细管电泳法的快速分离和定量能力使其成为辅助多重PCR优化过程的一种有效技术。
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling conditions, The process of optimizing a multiplex PCR reaction in order to obtain a well-balanced set of amplicons can be time-consuming and labor-intensive. The rapid separation and quantitation capabilities of capillary electrophoresis make it an efficient technique to help in the multiplex PCR optimization process.