Polysaccharide from the rhizomorph of Armillaria mella(AMP-1)protects INS-1 cells from alloxan injury
Polysaccharide from the rhizomorph of Armillaria mella(AMP-1)protects INS-1 cells from alloxan injury
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发表时间：
2008
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通讯作者：
Shen Ye-shou
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作者：
Shen Ye-shou
目的研究蜜环菌梅拉菌索多糖（AMP-1）对四氧嘧啶损伤的INS-1细胞的保护作用（2，10，50，100，500，在四氧嘧啶损伤的INS-1细胞培养液中加入葡萄糖（葡萄糖浓度为1000 mg·L-1），MTT法检测细胞存活率，葡萄糖浓度为1000 mg·L-1时，培养液中葡萄糖浓度为1000 mg·L-1用放射免疫分析法测定不同浓度AMP-1刺激胰岛素分泌的作用，结果AMP-1能明显减轻四氧嘧啶对INS-1细胞的氧化损伤，细胞存活率明显低于对照组（P < 0. 05）。在5.6mmol·L-1或16.7mmol·L-1葡萄糖存在下，AMP-1 AMP-1（50、100、500、1000mg·L-1）可剂量依赖性地增加葡萄糖诱导的INS-1细胞胰岛素分泌，降低NOS水平，减少NO和MDA的生成，结论AMP-1通过增强对四氧嘧啶所致自由基的清除能力，促进葡萄糖诱导的INS-1细胞胰岛素分泌。
Aim To investigate the protective effects of polysaccharides from the rhizomorph of Armillaria mella(AMP-1) on alloxan injured INS-1 cells.Methods Graded concentrations of AMP-1(2,10,50,100,500,1 000 mg·L-1) were added into the culture medium of alloxan injured INS-1 cells.The survival rate was measured by MTT assay.The amount of glucose-stimulated insulin secretion in different concentrations of AMP-1 was determined by radioimmunoassay(RIA).SOD and NOS activity,NO,MDA and GSH production were assayed colorimetrically.Results AMP-1 could reduce oxidative injuries induced by alloxan in INS-1 cells.The survival rate of cells treated with AMP-1 increased significantly.In the presence of 5.6 mmol·L-1 or 16.7 mmol·L-1 glucose,AMP-1(50,100,500,1 000 mg·L-1)increased glucose-induced insulin secretion in INS-1 cells in a dose-dependent manner.NOS levels and the production of NO and MDA decreased significantly by AMP-1,while SOD levels and the production of GSH increased.Conclusions AMP-1 promoted glucose-induced insulin secretion in INS-1 cells by increasing the abilities of scavenging the free radicals induced by alloxan.