Anthocyanin and carotenoid pigmentation in flowers of section Mina, subgenus Quamoclit, genus Ipomoea
Anthocyanin and carotenoid pigmentation in flowers of section Mina, subgenus Quamoclit, genus Ipomoea
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DOI：
10.1007/s10681-011-0618-4
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发表时间：
2012-01
期刊：
影响因子：
1.9
通讯作者：
C. Yamamizo;N. Noda;A. Ohmiya
中科院分区：
文献类型：
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作者：
C. Yamamizo;N. Noda;A. Ohmiya
植物属牡丹属植物中表现出特有的花瓣色素沉着。例如，何首乌的花瓣。黄体植物的颜色为黄色，来自类胡萝卜素，而黄体植物的颜色为红色，来自天竺葵花青素。本文对花瓣色素组成及色素生物合成基因的表达模式进行了研究。quamoclitandI。hederifoliavar。对黄体色素进行了分析，阐明了决定黄体切片花瓣颜色的关键因素。白花的花瓣。由于花青素合成酶（anthocyanidin synthase， ANS）基因表达受到抑制，而多种致胡萝卜素基因的转录下调，使得青茅缺乏花青素和类胡萝卜素的合成能力。在花瓣里。hederifoliavar。在叶黄素中，缺乏二氢黄酮醇4-还原酶（DFR）基因表达是花青素缺乏的原因。所有的f1子代都是通过种间杂交获得的。quamoclitandI。hederifoliavar。叶黄素的红色花瓣是由花青素和类胡萝卜素混合而成的。黄酮3-羟化酶基因、DFR基因和ANS基因在f1后代花瓣中表达。结果表明，在这些品种中，花瓣中的类胡萝卜素积累表型是显性的，白花的dfr在f1后代中是通过互补表达产生的。quamoclitandANSfromI。hederifoliavar.lutea。
Plants belong to the sectionMinashowed characteristic petal pigmentation amongIpomoeaplants. For example, petals ofIpomoea hederifoliavar.luteadisplay yellow color derived from carotenoids and those ofIpomoea quamoclitdisplay red color derived from pelargonidin-based anthocyanins. In this study, the pigment composition and the expression patterns of genes for pigment biosynthesis in the petals ofI. quamoclitandI. hederifoliavar.luteawere analyzed to elucidate the crucial factors that determine petal color in sectionMina. The petals of white-floweredI. quamoclitlack the ability to synthesize anthocyanins and carotenoids because of the suppression of anthocyanidin synthase (ANS) gene expression and transcriptional down-regulation of multiple carotenogenic genes, respectively. In petals ofI. hederifoliavar.lutea, the absence of dihydroflavonol 4-reductase (DFR) gene expression is responsible for the lack of anthocyanins. All F1progeny obtained by interspecies crossing betweenI. quamoclitandI. hederifoliavar.luteahad scarlet petal color produced by a combination of pelargonidin-based anthocyanins and carotenoids. The flavanone 3-hydroxylase gene, DFR, and ANS were expressed in the petals of the F1progeny. The results suggest that in these sectionMinaspecies, the carotenoid-accumulating phenotype in petals is dominant, and that pelargonidin was produced in the F1progeny by complementary expression ofDFRfrom white-floweredI. quamoclitandANSfromI. hederifoliavar.lutea.