Changes in soil microbial community structure with tillage under long-term wheat-fallow management
Changes in soil microbial community structure with tillage under long-term wheat-fallow management
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DOI：
10.1016/s0038-0717(00)00060-2
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发表时间：
2000-09-01
影响因子：
9.7
通讯作者：
Lyon, DJ
中科院分区：
文献类型：
--
作者：
Drijber, RA;Doran, JW;Lyon, DJ
脂肪酸甲酯（FAME）被用来“指纹”土壤微生物群落，在25年的小麦休耕后，在西德尼，内布拉斯加州，美国本土混合草原草皮。总酯连接FAME（EL-FAME）和磷脂连接FAME（PL-FAME）进行了比较，他们的能力，以区分地块剩余的草皮和那些种植小麦或休耕免耕，亚耕或犁管理。耕作地块的微生物生物量高于休耕，并且在0-15 cm深度的耕作中没有显著差异。休闲时，微生物量以免耕最大，翻耕最小。PL-和EL-FAME的聚类和判别分析清楚地将剩余的原生草皮地块与现有的小麦休闲地块分开。这种分离对于EL-FAME特别明显，并且在很大程度上是由单一FAME C16：1（顺式11）在sod中的高含量驱动的，其已被引用为丛枝菌根（AM）真菌的生物标志物。在小麦休闲，C16：1（cis 11）显着下降，从免耕犁，这支持的起源C16：1（cis 11）从根外的菌丝体和孢子的AM真菌已知是敏感的土壤扰动。虽然判别分析PL-和EL-FAMEs区分小麦和休闲系统耕作，耕作处理之间的歧视表示最强烈的休闲。FAME配置文件从休耕是最不同的作物土壤，这表明耕作管理和小麦作物下开发的微生物群落的长期弹性之间的关系。(C)2000爱思唯尔科技有限公司版权所有。
Fatty acid methyl esters (FAMEs) were used to 'fingerprint' soil microbial communities that evolved during 25 years of wheat-fallow cropping following native mixed prairie sod at Sidney, Nebraska, USA. Total ester-linked FAMEs (EL-FAMEs) and phospholipid-linked FAMEs (PL-FAMEs) were compared for their ability to discriminate between plots remaining in sod and those cropped to wheat or left fallow under no-till, sub-till or plow management. Cropped plots were higher in microbial biomass than their fallowed counterparts, and did not differ significantly with tillage for the 0-15 cm depth. Under fallow, microbial biomass was greatest in no-till and least in plow. Both cluster and discriminant analysis of PL- and EL-FAMEs clearly separated the remaining native sod plots from the existing wheat-fallow plots. This separation was particularly pronounced for the EL-FAMEs and was largely driven by high amounts in sod of a single FAME, C16:1(cis11), which has been cited as a biomarker for arbuscular mycorrhizal (AM) fungi. Within wheat-fallow, C16:1(cis11) declined significantly from no-till to plow, which supports the origin of C16:1(cis11) from extraradical mycelium and spores of AM fungi known to be sensitive to soil disturbance. Although discriminant analysis of PL- and EL-FAMEs differentiated wheat and fallow systems by tillage, discrimination among tillage treatments was expressed most strongly during fallow. FAME profiles from fallow plow were most dissimilar from cropped soils which suggests a relationship between tillage management and the long-term resiliency of the microbial community developed under the wheat crop. (C) 2000 Elsevier Science Ltd. All rights reserved.