Prognostic implications of ezrin and phosphorylated ezrin expression in non-small cell lung cancer.
Prognostic implications of ezrin and phosphorylated ezrin expression in non-small cell lung cancer.
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DOI：
10.1186/1471-2407-14-191
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发表时间：
2014-03-15
期刊：
影响因子：
3.8
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Jin T;Jin J;Li X;Zhang S;Choi YH;Piao Y;Shen X;Lin Z
细胞骨架组织者ezrin是ezrin-radixin-moesin（ERM）家族的成员，不仅在细胞运动、细胞粘附和凋亡中起重要作用，而且在各种细胞信号传导途径中也起重要作用。在Thr-567和Tyr-353的磷酸化是ezrin的休眠形式向活性形式转变的关键调节事件。本研究旨在探讨ezrin和磷酸化ezrin（p-ezrin）表达在非小细胞肺癌（NSCLC）中的预后意义。应用免疫组织化学方法检测150例NSCLC及癌旁组织和14例正常肺组织中Ezrin和p-Ezrin蛋白的表达。qRT-PCR用于测定新鲜组织中ezrin mRNA表达水平。分析ezrin和p-ezrin过表达与NSCLC临床病理特征的关系。采用Kaplan-Meier法计算108例NSCLC患者的生存率。Ezrin和ezrinThr-567蛋白显示胞质和膜染色模式;然而，ezrinTyr-353蛋白仅显示胞质染色。Ezrin和p-ezrin在NSCLC中与正常对照组相比显著上调。ezrin、ezrinThr-567和ezrinTyr-353水平升高与NSCLC的晚期和低分化相关。然而，只有ezrinThr-567与淋巴结转移的存在相关。在生存方面，只有ezrinThr-567与NSCLC患者的总生存时间相关，ezrin和ezrinThr-567均与早期NSCLC患者的生存时间缩短相关。Ezrin和p-Ezrin，尤其是ezrinThr-567，可能被证明是一种新的NSCLC预后生物标志物。
The cytoskeletal organizer ezrin is a member of the ezrin-radixin-moesin (ERM) family and plays important roles in not only cell motility, cell adhesion, and apoptosis, but also in various cell signaling pathways. Phosphorylation at Thr-567 and Tyr-353 are key regulatory events in the transition of the dormant to active form of ezrin. This study investigated the prognostic implications of ezrin and phosphorylated ezrin (p-ezrin) expression in non-small cell lung carcinoma (NSCLC). Ezrin and p-ezrin protein expressions were examined by immunohistochemistry in 150 NSCLC and adjacent non-tumor tissues and 14 normal lung tissues. qRT-PCR was used to determine ezrin mRNA expression levels in fresh tissues. The correlations between overexpression of ezrin and p-ezrin and the clinicopathological features of NSCLC were analyzed. The survival rates were calculated by the Kaplan-Meier method for 108 NSCLC cases. Ezrin and ezrinThr-567 proteins showed cytosolic and membranous staining patterns; however, ezrinTyr-353 protein only showed cytosolic staining. Ezrin and p-ezrin were significantly upregulated in NSCLC compared with the normal counterparts. Increased ezrin, ezrinThr-567, and ezrinTyr-353 levels were correlated with the late stage and poor differentiation of NSCLC. However, only ezrinThr-567 was correlated with the presence of lymph node metastasis. In regard to survival, only ezrinThr-567 was related with the overall survival time of patients with NSCLC, and both ezrin and ezrinThr-567 were associated with shortened survival time for patients with early stage NSCLC. Ezrin and p-ezrin, especially ezrinThr-567, may prove to be useful as a novel prognostic biomarker of NSCLC.
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