Laser photoacoustic detection allows in planta detection of nitric oxide in tobacco following challenge with avirulent and virulent Pseudomonas syringae pathovars
Laser photoacoustic detection allows in planta detection of nitric oxide in tobacco following challenge with avirulent and virulent Pseudomonas syringae pathovars
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DOI：
10.1104/pp.104.055772
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发表时间：
2005-07-01
期刊：
影响因子：
7.4
通讯作者：
Smith, AR
中科院分区：
文献类型：
--
作者：
Mur, LAJ;Santosa, IE;Smith, AR
我们证明了使用激光光声检测（LPAD）作为一种高灵敏度的方法来检测植物中的一氧化氮（.NO）的生产烟草（烟草）。LPAD校准。NO气体表现出超过2个数量级的线性关系，检测阈值< 20 pmol h（-1）（1 ppb体积）。光声信号的特异性。没有添加气体或。NO供体硝普钠注射到植物叶片中，被O-3（（NO）-N-.+）O-3 -> NO2+O-2）。LPAD方法的实用性通过检查非宿主过敏反应和由假单胞菌引起的疾病来显示。pv phaseolicola和P.s. pv tabaci in tobacco用P.s.攻击后40 min内检测到NO。菜豆生条斑病，在可见组织塌陷开始前约5小时。野火烟草病原体P.s.启动了烟粉虱病毒。感染后2 h有NO生成。使用. NO供体、清除剂CPTIO（[4-羧基苯基]-4，5-二氢-4，4，5，5-四甲基-3-氧化物）和哺乳动物一氧化氮合酶抑制剂L-NMMA（N-G-单甲基-L-精氨酸）表明。NO影响烟草细胞死亡动力学和对无毒和有毒细菌的抗性。这些观察表明。NO是与烟草中的这两种细菌病原体相关的细胞死亡的引发不可或缺的。
We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide (.NO) production from tobacco (Nicotiana tabacum). LPAD calibration against. NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshold of < 20 pmol h(-1) (1 part per billion volume [ppbv]). The specificity of the photoacoustic signal for. NO when adding gas or the. NO donor, sodium nitroprusside, on injection into plant leaves, was demonstrated by its abolition with O-3 ((NO)-N-.+O-3 -> NO2+O-2). The utility of the LPAD method was shown by examination of a nonhost hypersensitive response and a disease induced by Pseudomonas syringae (P. s.) pv phaseolicola and P. s. pv tabaci in tobacco.. NO was detected within 40 min of challenge with P. s. pv phaseolicola, some 5 h before the initiation of visible tissue collapse. The wildfire tobacco pathogen P. s. pv tabaci initiated. NO generation at 2 h postinfection. The use of. NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro- 4,4,5,5-tetramethyl-3- oxide), and the mammalian nitric oxide synthase inhibitor L- NMMA (N-G-monomethyl-L-arginine) indicated that. NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that. NO is integral to the elicitation of cell death associated with these two bacterial pathogens in tobacco.