Boosting mediated electron transfer in bioelectrochemical systems with tailored defined microbial cocultures
Boosting mediated electron transfer in bioelectrochemical systems with tailored defined microbial cocultures
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DOI：
10.1002/bit.26732
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发表时间：
2018-09-01
影响因子：
3.8
通讯作者：
Rosenbaum, Miriam A.
中科院分区：
文献类型：
--
作者：
Schmitz, Simone;Rosenbaum, Miriam A.
生物电化学系统（BES）通过微生物催化剂从有机物（例如，从废水）中产生可持续能源具有很大的前景。为了提高BES的电流生成，了解电极群落的基本微生物学是必不可少的。绿脓杆菌等产生电子介质的微生物在北京谱仪的高效发电中起着至关重要的作用。这些微生物甚至使非电活性微生物如产气肠杆菌也能为当前的生产做出贡献。他们一起形成了一个协同的共同文化，两者都有助于社区福利。要在BES中使用微生物合作，在共培养的自然栖息地中提供的物理和化学环境起着至关重要的作用。在这里，我们表明，在确定的铜绿假单胞菌和大肠杆菌共培养的协同效应。通过调整工艺参数，如pH、温度、需氧量和底物要求，可以大大提高产气菌的高电流生产能力。特别是，氧气被确定为影响共培养行为的主要因素，其供应的优化可以提高电流生产超过400%。此外，以补料分批模式操作共培养物使我们能够获得非常高的电流密度并收获1个月的电能。在此优化条件下，该过程的库仑效率提高到20%，这对于基于介体的电子转移是突出的。这项研究奠定了基础，合理设计利用共培养物在北京谱仪的生物能源发电从特定的废水或生物过程传感和受益于它们的协同效应，在受控的生物过程条件下。
Bioelectrochemical systems (BES) hold great promise for sustainable energy generation via a microbial catalyst from organic matter, for example, from wastewater. To improve current generation in BES, understanding the underlying microbiology of the electrode community is essential. Electron mediator producing microorganism like Pseudomonas aeruginosa play an essential role in efficient electricity generation in BES. These microbes enable even nonelectroactive microorganism like Enterobacter aerogenes to contribute to current production. Together they form a synergistic coculture, where both contribute to community welfare. To use microbial co-operation in BES, the physical and chemical environments provided in the natural habitats of the coculture play a crucial role. Here, we show that synergistic effects in defined cocultures of P. aeruginosa and E. aerogenes can be strongly enhanced toward high current production by adapting process parameters, like pH, temperature, oxygen demand, and substrate requirements. Especially, oxygen was identified as a major factor influencing coculture behavior and optimization of its supply could enhance electric current production over 400%. Furthermore, operating the coculture in fed-batch mode enabled us to obtain very high current densities and to harvest electrical energy for 1 month. In this optimized condition, the coulombic efficiency of the process was boosted to 20%, which is outstanding for mediator-based electron transfer. This study lays the foundation for a rationally designed utilization of cocultures in BES for bioenergy generation from specific wastewaters or for bioprocess sensing and for benefiting from their synergistic effects under controlled bioprocess condition.