Origin of noradrenergic projections to GnRH perikarya-containing areas in the medial septum-diagonal band and preoptic area.
Origin of noradrenergic projections to GnRH perikarya-containing areas in the medial septum-diagonal band and preoptic area.
复制标题
去甲肾上腺素能投射到内侧隔膜对角带和视前区中含有 GnRH 核周区域的起源。
DOI：
10.1016/0006-8993(93)90116-5
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Jennes,L
中科院分区：
文献类型：
--
作者：
Wright,DE;Jennes,L
由于去甲肾上腺素（NE）影响促性腺激素释放激素（GnRH）神经元的活性，本研究的目的是确定去甲肾上腺素能纤维投射到含有GnRH胞体的区域的起源部位。采用荧光逆行示踪法结合免疫组织化学法检测多巴胺-β-羟化酶（DBH）和GnRH。将小体积的荧光金（FG）或荧光红宝石（FR）压力注射到含有最大数量的GnRH细胞体的区域，即，内侧隔-斜角带复合体或视前区。逆行标记神经元观察同侧以下去甲肾上腺素能细胞群：A2（在孤束核），A1（在延髓腹外侧）和蓝斑。A2细胞组中约8%的DBH阳性神经元被逆行标记，而A1细胞组中12%的DBH阳性神经元被双标记。蓝斑内仅观察到少量逆行标记的DBH阳性神经元（<1%）。双标记的神经元没有组织成离散的细胞群，但分散在其他NE-神经元内的A2和A1细胞组。最高浓度的双标记神经元位于中央三分之一的A2和A1细胞组。结果提示，内侧隔-斜角带/吻侧视前区GnRH胞体内的去甲肾上腺素能终末主要来源于同侧A1和A2区神经元。这些数据支持这样的观点，即髓质细胞群A1和A2，而不是蓝斑，是去甲肾上腺素能成分的主要来源，这是至关重要的直接调节GnRH胞体。
The purpose of the present study was to identify the sites of origin of the noradrenergic fibers that project to areas containing gonadotropin-releasing hormone (GnRH) perikarya since norepinephrine (NE) is known to influence the activity of GnRH neurons. Fluorescent retrograde tracers were used in combination with immunohistochemistry for dopamine-β-hydroxylase (DBH) and GnRH. Small volumes of either Fluoro-gold (FG) or Fluoro-Ruby (FR) were pressure injected into areas that contain the largest number of GnRH cell bodies, i.e., the medial septum-diagonal band complex or preoptic area. Retrogradely labeled neurons were observed ipsilaterally in the following noradrenergic cell groups: A2 (in the nucleus tractus solitarii), A1 (in the ventrolateral medulla) and locus coeruleus. Approximately 8% of all DBH-positive neurons within the A2-cell group were retrogradely labeled, while 12% of DBH-ir neurons in the A1-group were double-labeled. Only a few retrogradely labeled DBH-ir neurons were observed in the locus coeruleus (<1%). Double-labeled neurons were not organized into discrete cell groups, but were dispersed among other NE-neurons within the A2- and A1-cell groups. The highest concentrations of double-labeled neurons were located in the central one-third of both the A2 and A1 cell groups. The results suggest that most noradrenergic terminals in the region of the GnRH perikarya in the medial septum-diagonal band/rostral preoptic area originate from ipsilateral neurons in areas A1 and A2. These data support the view that the medullary cell groups A1 and A2, and not the locus coeruleus, are the primary sites of origin of the noradrenergic component that is crucial for the direct regulation of GnRH perikarya.