CORONARY-ARTERY DEVELOPMENT IN THE CHICK - ORIGIN AND DEPLOYMENT OF SMOOTH-MUSCLE CELLS, AND THE EFFECTS OF NEURAL CREST ABLATION
CORONARY-ARTERY DEVELOPMENT IN THE CHICK - ORIGIN AND DEPLOYMENT OF SMOOTH-MUSCLE CELLS, AND THE EFFECTS OF NEURAL CREST ABLATION
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DOI：
10.1002/ar.1092340215
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发表时间：
1992-10-01
影响因子：
--
通讯作者：
ROSENQUIST, TH
中科院分区：
文献类型：
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作者：
HOOD, LC;ROSENQUIST, TH
先前对冠状动脉个体发育的研究强调早期发育，因此主要关注新生冠状动脉干内皮的起源。本研究通过确定平滑肌α - 肌动蛋白（SMAA）出现的时间和分布，分析了家鸡冠状动脉中血管平滑肌细胞（VSMC）的个体发育。将抗 - SMAA应用于正常胚胎切片以及在体节1 - 3处接受神经嵴手术切除的实验胚胎切片。结果显示，在对照冠状动脉中SMAA呈有序对称分布。与心脏流出血管的平滑肌细胞相比，冠状动脉VSMC中SMAA的表达明显更早；这种早期表达可能表明对平滑肌表型诱导的独特反应性。冠状动脉VSMC的正常有序发育依赖于神经嵴的存在，因此在神经嵴被切除的实验胚胎中其发育受到干扰。
Previous studies of coronary artery ontogeny have stressed early development and therefore have dwelt mainly upon the origin of the endothelium of the nascent coronary artery stem. This study has analyzed the ontogeny of the vascular smooth muscle cells (VSMC) in the coronary arteries of the domestic chicken, by establishing the timing and deployment of smooth muscle alpha-actin (SMAA). Anti-SMAA was applied to sections of normal embryos, and to sections of experimental embryos that had undergone surgical ablation of the neural crest over somites 1-3. The results show an orderly symmetrical deployment of SMAA in control coronary arteries. SMAA was expressed significantly earlier in the coronary artery VSMC compared with those of the cardiac outflow vessels; this early expression may indicate a unique responsiveness to induction of the smooth muscle phenotype. The normal orderly development of coronary artery VSMC was dependent upon the presence of the neural crest, and therefore was disrupted in the experimental embryos whose neural crest was ablated.