Autonomous reagent-based microfluidic pH sensor platform
Autonomous reagent-based microfluidic pH sensor platform
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DOI：
10.1016/j.snb.2015.11.057
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发表时间：
2016-03-31
影响因子：
8.4
通讯作者：
Diamond, Dermot
中科院分区：
文献类型：
--
作者：
Sansalvador, Isabel M. Perez de Vargas;Fay, Cormac D.;Diamond, Dermot
开发了一种便携式传感器，用于在水环境中原位测量pH值。传感器设计结合了微流体技术，允许使用小体积的样品和试剂，以及一个集成的低成本检测系统，使用发光二极管作为光源和光电二极管作为检测器。不同的染料组合已被研究，以允许更广泛的pH值检测范围，可以获得比使用单一染料。发现这种特殊染料组合的最佳pH范围在pH 4和pH 9之间。开发的pH测量试剂首先使用台式仪器进行测试，一旦优化，选定的配方就会在微流体系统中实施。该原型系统在pH响应、线性范围、可重复性和稳定性方面进行了表征。使用原型系统获得的结果与使用参考仪器（即玻璃电极/pH计和基于分光光度计的分析）获得的结果非常一致。该试剂（混合物#3）的稳定性超过8个月，这对于长期部署非常重要。该方法重现性高，总体RSD为
A portable sensor has been developed for in situ measurements of pH within aqueous environments.The sensor design incorporates microfluidic technology, allowing for the use of low volume of samples and reagents, and an integrated low cost detection system that uses a light emitting diode as light source and a photodiode as the detector. Different combination of dyes has been studied in order to allow for a broader pH detection range, than can be obtained using a single dye. The optimum pH range for this particular dye combination was found to be between pH 4 and pH 9.The reagents developed for pH measurement were first tested using bench-top instrumentation and once optimised, the selected formulation was then implemented in the microfluidic system.The prototype system has been characterised in terms of pH response, linear range, reproducibility and stability. Results obtained using the prototype system are in good agreement with those obtained using reference instrumentation, i.e. a glass electrode/pH meter and analysis via spectrophotometer based assays.The reagent (mixture #3) is shown to be stable for over 8 months, which is important for long term deployments. A high reproducibility is reported with a global RSD of