Antidepressant effect and pharmacological evaluation of standardized extract of flavonoids from Byrsonima crassifolia
Antidepressant effect and pharmacological evaluation of standardized extract of flavonoids from Byrsonima crassifolia
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DOI：
10.1016/j.phymed.2011.06.018
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发表时间：
2011-11-15
期刊：
影响因子：
7.9
通讯作者：
Huerta-Reyes, M.
中科院分区：
文献类型：
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作者：
Herrera-Ruiz, M.;Zamilpa, A.;Huerta-Reyes, M.
Byrsonima crassifolia（金虎尾科）已在传统医学中用于治疗一些与精神相关的疾病：然而，其具体的神经药理学活性仍有待确定。本研究评估了 Byrsonima crassifolia 提取物产生的抗焦虑、抗惊厥、抗抑郁、镇静作用及其对 ICR 小鼠运动活动的影响。此外，我们还确定了 Byrsonima crassifolia 提取物的急性毒性特征以及神经活性成分的存在。我们的结果表明，在小鼠强迫游泳试验中，500 mg/kg 剂量的 Byrsonima crassifolia 甲醇提取物产生显着的（P < 0.05）抗抑郁作用。然而，它不具有抗焦虑、镇静或抗惊厥特性，并且不会导致小鼠运动能力下降（P>0.05）。尽管甲醇提取物的主要化合物被鉴定为槲皮素 3-O-木糖苷（12 毫克/千克），但我们的发现；表明类黄酮，如芦丁（4.4 毫克/千克）、槲皮素（1.4 毫克/千克）和橙皮苷（0.7 毫克/千克），可能参与抗抑郁作用。据我们所知，本研究是第一份关于 Byrsonima crassifolia 中存在具有神经药理学活性的黄酮类化合物芦丁和橙皮苷的报告。总之，本研究结果表明，以青叶黄酮含量标准化的甲醇提取物在小鼠 FST 中具有潜在的抗抑郁样作用，并且在毒理学上可以认为相对安全，口服 2000 mg/kg 时没有小鼠死亡。 (C) 2011 爱思唯尔有限公司。版权所有。
Byrsonima crassifolia (Malpighiaceae) has been used in traditional medicine for the treatment of some mental-related diseases: however, its specific neuropharmacological activities remain to be defined. The present study evaluates the anxiolytic, anticonvulsant, antidepressant, sedative effects produced by the extracts of Byrsonima crassifolia, and their influence on motor activity in ICR mice. Additionally, we determine the acute toxicity profiles of the Byrsonima crassifolia extracts and the presence of neuroactive constituents. Our results show that the methanolic extract of Byrsonima crassifolia produces a significant (P< 0.05) antidepressant effect in the forced swimming test in mice at 500 mg/kg dose. However, it does not possess anxiolytic, sedative, or anticonvulsant properties, and does not cause a reduction of mice locomotion (P> 0.05). Although the main compound of the methanolic extract was identified as quercetin 3-O-xyloside (12 mg/kg), our findings; suggest that flavonoids, such as rutin (4.4 mg/kg), quercetin (1.4 mg/kg) and hesperidin (0.7 mg/kg), may be involved in the antidepressant effects. To the best of our knowledge, the present study constitutes the first report on the presence of the flavonoids with neuropharmacological activity rutin and hesperidin in Byrsonima crassifolia. In conclusion, the present results showed that the methanolic extract standardized on flavonoids content of Byrsonima crassifolia possesses potential antidepressant-like effects in the FST in mice, and could be considered as relatively safe toxicologically with no deaths of mice when orally administered at 2000 mg/kg. (C) 2011 Elsevier GmbH. All rights reserved.