S-Variant SARS-CoV-2 Lineage B1.1.7 Is Associated With Significantly Higher Viral Load in Samples Tested by TaqPath Polymerase Chain Reaction
S-Variant SARS-CoV-2 Lineage B1.1.7 Is Associated With Significantly Higher Viral Load in Samples Tested by TaqPath Polymerase Chain Reaction
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DOI：
10.1093/infdis/jiab082
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发表时间：
2021-05-04
影响因子：
6.4
通讯作者：
McNally, Alan
中科院分区：
文献类型：
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作者：
Kidd, Michael;Richter, Alex;McNally, Alan
含有突变Delta 69/70的SARS-CoV-2变体B1.1.7在英国迅速传播，并在ThermoFisher TaqPath RT-qPCR， S基因靶失败（SGTF）中显示出可识别的特征。我们分析了最近的测试数据的趋势和意义。呼吸样本的连接周期阈值（Ct）值显示，orf1ab和N的低Ct与SGTF明显相关。在1x10(7)和1x10(8)之间，更多的SGTF样本具有更高的推断病毒载量。我们的结论是，样本显示SGTF特征的患者更有可能具有高病毒载量，这可能解释了更高的传染性和传播速度。
A SARS-CoV-2 variant B1.1.7 containing mutation Delta 69/70 has spread rapidly in the United Kingdom and shows an identifiable profile in ThermoFisher TaqPath RT-qPCR, S gene target failure (SGTF). We analyzed recent test data for trends and significance. Linked cycle threshold (Ct) values for respiratory samples showed that a low Ct for ORF 1 ab and N were clearly associated with SGTF. Significantly more SGTF samples had higher inferred viral loads between 1x10(7) and 1x10(8). Our conclusion is that patients whose samples exhibit the SGTF profile are more likely to have high viral loads, which may explain higher infectivity and rapidity of spread.