Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors
Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors
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低拷贝和高拷贝转化载体上盘基网柄菌肌动蛋白基因融合体的发育调控
DOI：
10.1128/mcb.6.11.3973-3983.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
H. Lodish
中科院分区：
文献类型：
--
作者：
D. Knecht;S. Cohen;W. Loomis;H. Lodish
盘基网柄藻的基因组含有大约17到20个肌动蛋白基因。我们报告的鉴定，这个多基因家族的一个新成员，肌动蛋白15，其完整的核苷酸序列和转录起始位点。我们构建了携带肌动蛋白15启动子融合的转座子Tn903的新霉素磷酸转移酶基因或肌动蛋白6启动子融合的Tn5的新霉素磷酸转移酶基因的转化载体。用肌动蛋白15载体转化的细胞携带少于5个拷贝的载体DNA，而用肌动蛋白6载体转化的细胞携带超过200个拷贝。在这两种情况下，载体似乎作为串联阵列整合到染色体中。从actin 15和actin 6载体转录的基因融合RNA在D.盘状突发育这些基因的时间和细胞类型特异性调控所需的DNA序列包含在肌动蛋白15的5'非编码DNA的2.8个碱基和肌动蛋白6的5'非编码DNA的0.7个碱基内。
The Dictyostelium discoideum genome contains an estimated 17 to 20 actin genes. We report the identification of a new member of this multigene family, actin 15, and its complete nucleotide sequence and transcription initiation sites. We constructed transformation vectors carrying either the actin 15 promoter fused to the neomycin phosphotransferase gene from transposon Tn903 or the actin 6 promoter fused to the neomycin phosphotransferase gene from Tn5. Cells transformed with the actin 15 vector carried less than five copies of vector DNA, while cells transformed with the actin 6 vector carried more than 200 copies. In both cases, the vector appeared to be integrated into the chromosome as a tandem array. Gene fusion RNAs transcribed from the actin 15 and actin 6 vectors were regulated like endogenous actin genes during D. discoideum development. DNA sequences required for temporal and cell type-specific regulation of these genes were contained within 2.8 kilobases of 5' noncoding DNA for actin 15 and 0.7 kilobases of 5' noncoding DNA for actin 6.
通过比较 3 末端序列确定网柄菌中肌动蛋白基因亚家族的证据。
影响因子：
5.6
作者：
McKeown,M;Firtel,RA
通讯作者：
Firtel,RA