Nonuniform temporal alignment of slice sequences for four-dimensional imaging of cyclically deforming embryonic structures
Nonuniform temporal alignment of slice sequences for four-dimensional imaging of cyclically deforming embryonic structures
复制标题
用于循环变形胚胎结构四维成像的切片序列的非均匀时间对齐
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
S. Fraser
中科院分区：
文献类型：
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作者：
M. Liebling;J. Vermot;A. Forouhar;M. Gharib;M. Dickinson;S. Fraser
对在活的斑马鱼胚胎心脏的不同轴向位置获取的非标记化切片序列的时间比对允许重建动态的三维数据。这种方法克服了目前共焦显微镜对快速过程的实时三维成像的采集速度限制。当前的同步方法在时间上对齐并统一缩放数据，但不能补偿心跳中发生的心跳节奏的微小变化。因此，它们对可接受的数据质量施加了限制。在这里，我们推导了一种基于最小化相邻切片-序列对之间的亮度差的绝对值的非均匀配准过程。该方法补偿了样本内的时间变化，并允许对更大范围的数据进行处理，以建立跳动的胚胎心脏的功能动态模型。我们展示了从活的、荧光斑马鱼胚胎中获得的数据的重建
The temporal alignment of nongated slice-sequences acquired at different axial positions in the living embryonic zebrafish heart permits the reconstruction of dynamic, three-dimensional data. This approach overcomes the current acquisition-speed limitation of confocal microscopes for real-time three-dimensional imaging of fast processes. Current synchronization methods align and uniformly scale the data in time, but do not compensate for slight variations in the heart rhythm that occur within a heartbeat. Therefore, they impose constraints on the admissible data quality. Here, we derive a nonuniform registration procedure based on the minimization of the absolute value of the intensity difference between adjacent slice-sequence pairs. The method compensates for temporal intra-sample variations and allows the processing of a wider range of data to build functional, dynamic models of the beating embryonic heart. We show reconstructions from data acquired in living, fluorescent zebrafish embryos